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• W2182124342 abstract "A B S T RA C T The effect of intestinal bacterial overgrowth on brush border hydrolases and brush border glycoproteins was studied in nonoperated control rats, control rats with surgically introduced jejunal selfemptying blind loops, and rats with surgically introduced jejunal self-filling blind loops. Data were analyzed from blind loop segments, segments above and below the blind loops, and three corresponding segments in the nonoperated controls. Rats with selffilling blind loops had significantly greater fat excretion than controls and exhibited significantly lower conjugated:free bile salt ratios in all three segments. Maltase, sucrase, and lactase activities were significantly reduced in homogenates and isolated brush borders from the self-filling blind loop, but alkaline phosphatase was not affected. The relative degradation rate of homogenate and brush border glycoproteins was assessed by a double-isotope technique involving the injection of D-[6-3H]glucosamine 3 h and D[U-14C]glucosamine 19 h before sacrifice, and recorded as a 3H: 14C ratio. The relative degradation rate in both homogenate and brush border fractions was significantly greater in most segments from rats with selffilling blind loops. In the upper and blind loop segments from rats with self-filling blind loops, the 3H:14C ratios were higher in the brush border membrane than in the corresponding homogenates, indicating that the increased rates of degradation primarily involve membrane glycoproteins. Incorporation of D-[6-3H]glucosamine by brush border glycoproteins" @default.
• W2182124342 created "2016-06-24" @default.
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• W2182124342 date "1977-01-01" @default.
• W2182124342 modified "2023-09-27" @default.
• W2182124342 title "BRUSH BORDER ENZYME ACTIVITY AND GLYCOPROTEIN DEGRADATION" @default.
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