Matches in SemOpenAlex for { <https://semopenalex.org/work/W2182155272> ?p ?o ?g. }
- W2182155272 abstract "Electron microscopy investigations require excellent ultrastructure preservation, coupled with high antigenicity signal in specific applications of Golgi enzyme localization. The optimal detection of membrane-bound proteins needs appropriate specimen preparation techniques, such as cryo-methods known to provide higher cell structure and antigen preservation than conventional techniques. Two cryopreparation methods (high-pressure freezing and Tokuyasu techniques) applied to tobacco cultured cell lines are presented in this study. Two transgenic lines of tobacco cultured cells expressing GFP-fused proteins (Man-I-GFP and MUR3-GFP) were used to assess antigenicity under various conditions of cryomethods. According to cell morphology and immunolabeling detection, pre-freezing treatments are discussed in detail for high pressure freezing and freeze substituted samples embedded in both Spurr’s and London Resin White. In a same approach, the effect of two fixative solutions was investigated for cryosectioned suspensioncultured tobacco cells using Tokuyasu technique. Integrity during the thawing step and subsequent immunolabeling were analysed under chemical fixation conditions." @default.
- W2182155272 created "2016-06-24" @default.
- W2182155272 creator A5017176019 @default.
- W2182155272 creator A5025977094 @default.
- W2182155272 creator A5033982052 @default.
- W2182155272 creator A5053869016 @default.
- W2182155272 creator A5076038396 @default.
- W2182155272 creator A5076848995 @default.
- W2182155272 date "2011-01-01" @default.
- W2182155272 modified "2023-10-06" @default.
- W2182155272 title "Cryopreparation of tobacco Bright Yellow-2 (BY-2) suspension-cultured cells and application to immunogold localization of Green Fluorescent protein-tagged-membrane proteins" @default.
- W2182155272 cites W1494075612 @default.
- W2182155272 cites W1532431243 @default.
- W2182155272 cites W1534149735 @default.
- W2182155272 cites W1612283417 @default.
- W2182155272 cites W1924532079 @default.
- W2182155272 cites W1925872210 @default.
- W2182155272 cites W1965247392 @default.
- W2182155272 cites W1966445035 @default.
- W2182155272 cites W1970227360 @default.
- W2182155272 cites W1981486057 @default.
- W2182155272 cites W1998927244 @default.
- W2182155272 cites W2005518160 @default.
- W2182155272 cites W2007402555 @default.
- W2182155272 cites W2016181161 @default.
- W2182155272 cites W2018134701 @default.
- W2182155272 cites W2021101258 @default.
- W2182155272 cites W2022131186 @default.
- W2182155272 cites W2032320777 @default.
- W2182155272 cites W2043597653 @default.
- W2182155272 cites W2044227222 @default.
- W2182155272 cites W2045283994 @default.
- W2182155272 cites W2045715086 @default.
- W2182155272 cites W2056673093 @default.
- W2182155272 cites W2057240303 @default.
- W2182155272 cites W2063057981 @default.
- W2182155272 cites W2066844162 @default.
- W2182155272 cites W2067686807 @default.
- W2182155272 cites W2077535658 @default.
- W2182155272 cites W2084641320 @default.
- W2182155272 cites W2086233637 @default.
- W2182155272 cites W2098184049 @default.
- W2182155272 cites W2108563945 @default.
- W2182155272 cites W2108675560 @default.
- W2182155272 cites W2114093024 @default.
- W2182155272 cites W2118318984 @default.
- W2182155272 cites W2118474474 @default.
- W2182155272 cites W2119556473 @default.
- W2182155272 cites W2124100949 @default.
- W2182155272 cites W2124663138 @default.
- W2182155272 cites W2126961090 @default.
- W2182155272 cites W2132205053 @default.
- W2182155272 cites W2132396512 @default.
- W2182155272 cites W2132733262 @default.
- W2182155272 cites W2137638369 @default.
- W2182155272 cites W2140922007 @default.
- W2182155272 cites W2141488468 @default.
- W2182155272 cites W2150828550 @default.
- W2182155272 cites W2151001790 @default.
- W2182155272 cites W2155759845 @default.
- W2182155272 cites W2156757588 @default.
- W2182155272 cites W2157526623 @default.
- W2182155272 cites W2157827172 @default.
- W2182155272 cites W2161361416 @default.
- W2182155272 cites W2163773137 @default.
- W2182155272 cites W2165840965 @default.
- W2182155272 cites W2169489226 @default.
- W2182155272 cites W2336302230 @default.
- W2182155272 cites W2478830361 @default.
- W2182155272 cites W265817920 @default.
- W2182155272 cites W3003781173 @default.
- W2182155272 cites W644669495 @default.
- W2182155272 hasPublicationYear "2011" @default.
- W2182155272 type Work @default.
- W2182155272 sameAs 2182155272 @default.
- W2182155272 citedByCount "1" @default.
- W2182155272 countsByYear W21821552722012 @default.
- W2182155272 crossrefType "journal-article" @default.
- W2182155272 hasAuthorship W2182155272A5017176019 @default.
- W2182155272 hasAuthorship W2182155272A5025977094 @default.
- W2182155272 hasAuthorship W2182155272A5033982052 @default.
- W2182155272 hasAuthorship W2182155272A5053869016 @default.
- W2182155272 hasAuthorship W2182155272A5076038396 @default.
- W2182155272 hasAuthorship W2182155272A5076848995 @default.
- W2182155272 hasConcept C104317684 @default.
- W2182155272 hasConcept C105341887 @default.
- W2182155272 hasConcept C120665830 @default.
- W2182155272 hasConcept C121332964 @default.
- W2182155272 hasConcept C140051345 @default.
- W2182155272 hasConcept C142613039 @default.
- W2182155272 hasConcept C144647389 @default.
- W2182155272 hasConcept C185592680 @default.
- W2182155272 hasConcept C202444582 @default.
- W2182155272 hasConcept C30030713 @default.
- W2182155272 hasConcept C33923547 @default.
- W2182155272 hasConcept C41625074 @default.
- W2182155272 hasConcept C55493867 @default.
- W2182155272 hasConcept C5961521 @default.
- W2182155272 hasConcept C59822182 @default.
- W2182155272 hasConcept C86803240 @default.