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- W2182175848 abstract "PCR was used to detect Mycobacterium tuberculosis (MTB) complex and Mycobacterium bovis in local bovine milk samples. Along with PCR a single intradermal tuberculin test was also carried out for comparing the PCR with tuberculin test. In the case of MTB complex, a 984 bp fragment of insertion sequence IS6110 was amplified in TB positive cases while for M. bovis a 500 bp genomic fragment was amplified in milk samples. The multiplex PCR was also done by using both sets of primers specific to MTB complex and M. bovis. The incidence of MTB complex and M. bovis was found to be 11(35%) and 9(29%) respectively by PCR in milk samples out of total 31 samples, collected from buffaloes suspected for TB, as against 3 (9.6 %) cases of bovine TB after single intradermal tuberculin test." @default.
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- W2182175848 date "2008-06-04" @default.
- W2182175848 modified "2023-09-26" @default.
- W2182175848 title "Reliability of PCR for Detection of Bovine Tuberculosis in Pakistan" @default.
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