FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2182177316> ?p ?o ?g. }

• W2182177316 endingPage "491" @default.
• W2182177316 startingPage "483" @default.
• W2182177316 abstract "ABSTRACT Plectin, the largest and most versatile member of the cytolinker/plakin family of proteins characterized to date, has a tripartite structure comprising a central 200 nm-long α-helical rod domain flanked by large globular domains. The C-terminal domain comprises a short tail region preceded by six highly conserved repeats (each 28-39 kDa), one of which (repeat 5) contains plectin’s intermediate filament (IF)-binding site. We used recombinant and native proteins to assess the effects of plectin repeat 5-binding to IF proteins of different types. Quantitative Eu3+-based overlay assays showed that plectin’s repeat 5 domain bound to type III IF proteins (vimentin) with preference over type I and II cytokeratins 5 and 14. The ability of both types of IF proteins to self-assemble into filaments in vitro was impaired by plectin’s repeat 5 domain in a concentration- dependent manner, as revealed by negative staining and rotary shadowing electron microscopy. This effect was much more pronounced in the case of vimentin compared to cytokeratins 5/14. Preassembled filaments of both types became more and more crosslinked upon incubation with increasing concentrations of plectin repeat 5. However, at high proportions of plectin to IF proteins, disassembly of filaments occurred. Again, vimentin filaments proved considerably more sensitive towards disassembly than those composed of cytokeratins 5 and 14. In general, IFs formed from recombinant proteins were found to be slightly more responsive towards plectin influences than their native counterparts. A dose-dependent plectin- inflicted collapse and putative disruption of IFs was also observed in vivo after ectopic expression of vimentin and plectin’s repeat 5 domain in cotransfected vimentin- deficient SW13 (vim−) cells. Our results suggest an involvement of plectin not only in crosslinking and stabilization of cytoskeletal IF networks, but also in regulation of their dynamics." @default.
• W2182177316 created "2016-06-24" @default.
• W2182177316 creator A5014504040 @default.
• W2182177316 creator A5033520180 @default.
• W2182177316 creator A5069626671 @default.
• W2182177316 creator A5074920093 @default.
• W2182177316 creator A5089203723 @default.
• W2182177316 creator A5090346756 @default.
• W2182177316 date "2000-02-01" @default.
• W2182177316 modified "2023-10-12" @default.
• W2182177316 title "Dose-dependent linkage, assembly inhibition and disassembly of vimentin and cytokeratin 5/14 filaments through plectin’s intermediate filament-binding domain" @default.
• W2182177316 cites W1550465180 @default.
• W2182177316 cites W1631620978 @default.
• W2182177316 cites W1645438335 @default.
• W2182177316 cites W1646941628 @default.
• W2182177316 cites W1963659992 @default.
• W2182177316 cites W1965372133 @default.
• W2182177316 cites W1969117394 @default.
• W2182177316 cites W1969281285 @default.
• W2182177316 cites W1972502686 @default.
• W2182177316 cites W1982915032 @default.
• W2182177316 cites W1984610550 @default.
• W2182177316 cites W2003036450 @default.
• W2182177316 cites W2003434471 @default.
• W2182177316 cites W2010974498 @default.
• W2182177316 cites W2018661276 @default.
• W2182177316 cites W2023519337 @default.
• W2182177316 cites W2024764852 @default.
• W2182177316 cites W2024792219 @default.
• W2182177316 cites W2027583917 @default.
• W2182177316 cites W2032584413 @default.
• W2182177316 cites W2038835879 @default.
• W2182177316 cites W2039741195 @default.
• W2182177316 cites W2047607108 @default.
• W2182177316 cites W2050412379 @default.
• W2182177316 cites W2050549657 @default.
• W2182177316 cites W2058261637 @default.
• W2182177316 cites W2066390328 @default.
• W2182177316 cites W2070280831 @default.
• W2182177316 cites W2089271523 @default.
• W2182177316 cites W2089433310 @default.
• W2182177316 cites W2098254794 @default.
• W2182177316 cites W2105146516 @default.
• W2182177316 cites W2105350292 @default.
• W2182177316 cites W2107726648 @default.
• W2182177316 cites W2115034317 @default.
• W2182177316 cites W2122412289 @default.
• W2182177316 cites W2124751970 @default.
• W2182177316 cites W2126048177 @default.
• W2182177316 cites W2146629575 @default.
• W2182177316 cites W2161819130 @default.
• W2182177316 cites W2218922061 @default.
• W2182177316 doi "https://doi.org/10.1242/jcs.113.3.483" @default.
• W2182177316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10639335" @default.
• W2182177316 hasPublicationYear "2000" @default.
• W2182177316 type Work @default.
• W2182177316 sameAs 2182177316 @default.
• W2182177316 citedByCount "62" @default.
• W2182177316 countsByYear W21821773162012 @default.
• W2182177316 countsByYear W21821773162013 @default.
• W2182177316 countsByYear W21821773162014 @default.
• W2182177316 countsByYear W21821773162015 @default.
• W2182177316 countsByYear W21821773162016 @default.
• W2182177316 countsByYear W21821773162018 @default.
• W2182177316 countsByYear W21821773162022 @default.
• W2182177316 crossrefType "journal-article" @default.
• W2182177316 hasAuthorship W2182177316A5014504040 @default.
• W2182177316 hasAuthorship W2182177316A5033520180 @default.
• W2182177316 hasAuthorship W2182177316A5069626671 @default.
• W2182177316 hasAuthorship W2182177316A5074920093 @default.
• W2182177316 hasAuthorship W2182177316A5089203723 @default.
• W2182177316 hasAuthorship W2182177316A5090346756 @default.
• W2182177316 hasConcept C12554922 @default.
• W2182177316 hasConcept C14228908 @default.
• W2182177316 hasConcept C142669718 @default.
• W2182177316 hasConcept C147447768 @default.
• W2182177316 hasConcept C1491633281 @default.
• W2182177316 hasConcept C153911025 @default.
• W2182177316 hasConcept C203014093 @default.
• W2182177316 hasConcept C204232928 @default.
• W2182177316 hasConcept C2776666470 @default.
• W2182177316 hasConcept C2779012181 @default.
• W2182177316 hasConcept C2909370275 @default.
• W2182177316 hasConcept C54355233 @default.
• W2182177316 hasConcept C55493867 @default.
• W2182177316 hasConcept C78383274 @default.
• W2182177316 hasConcept C81241287 @default.
• W2182177316 hasConcept C86803240 @default.
• W2182177316 hasConcept C95444343 @default.
• W2182177316 hasConceptScore W2182177316C12554922 @default.
• W2182177316 hasConceptScore W2182177316C14228908 @default.
• W2182177316 hasConceptScore W2182177316C142669718 @default.
• W2182177316 hasConceptScore W2182177316C147447768 @default.
• W2182177316 hasConceptScore W2182177316C1491633281 @default.
• W2182177316 hasConceptScore W2182177316C153911025 @default.
• W2182177316 hasConceptScore W2182177316C203014093 @default.
• W2182177316 hasConceptScore W2182177316C204232928 @default.
• W2182177316 hasConceptScore W2182177316C2776666470 @default.

• next