FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2182177768> ?p ?o ?g. }

• W2182177768 endingPage "184" @default.
• W2182177768 startingPage "178" @default.
• W2182177768 abstract "Background Decreased cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride (Cl) secretion across mucosal surfaces contributes to the development of airway disease by depleting airway surface liquid, increasing mucus viscosity and adhesion, and consequently hindering mucociliary clearance. We serendipitously discovered during testing of drugs solubilized in low concentrations ethanol (0.25%, 43 mM) that the control vehicle produced robust activation of CFTR-mediated Cl− transport. The objective of the current study is to investigate low concentrations of ethanol for effects on Cl− secretion and ciliary beat frequency (CBF). Methods Wild-type (WT) and transgenic CFTR−/− primary murine nasoseptal epithelial (MNSE) cultures and WT and F508del/F508del human sinonasal epithelial (HSNE) cultures were subjected to transepithelial ion transport measurements using pharmacologic manipulation in Ussing chambers. CBF activation was also monitored. Murine nasal potential difference (NPD) was measured in vivo. Results Ussing chamber tracings revealed ethanol activated CFTR-mediated Cl transport in a dose-dependent fashion in WT MNSE (n = 4, p < 0.05) and HSNE (n = 4, p < 0.05). Ethanol also significantly increased CBF (fold change) in WT MNSE cultures in a dose-dependent fashion (phosphate-buffered saline [PBS], 1.33 ± 0.04; 0.25% ethanol, 1.37 ± 0.09; 0.5% ethanol, 1.53 ± 0.06 [p < 0.05]; 1% ethanol, 1.62 ± 0.1 [p < 0.05]). Lack of stimulation in CFTR−/− and F508del/F508del cultures indicated activity was dependent on the presence of intact functional CFTR. Ethanol perfusion (0.5%) resulted in a significant −3.5-mV mean NPD polarization when compared to control solution (p < 0.05). Conclusion The observation that brief exposure of ethanol stimulated Cl− secretion via CFTR-mediated pathways indicates its possible use as topical aerosol delivered alone or in combination with other CFTR activators for diseases of dysfunctional mucociliary clearance (MCC) in chronic rhinosinusitis (CRS)." @default.
• W2182177768 created "2016-06-24" @default.
• W2182177768 creator A5026337502 @default.
• W2182177768 creator A5035736153 @default.
• W2182177768 creator A5036733807 @default.
• W2182177768 creator A5050704941 @default.
• W2182177768 creator A5050727273 @default.
• W2182177768 creator A5059406657 @default.
• W2182177768 creator A5061587101 @default.
• W2182177768 date "2015-12-03" @default.
• W2182177768 modified "2023-09-23" @default.
• W2182177768 title "Cystic fibrosis transmembrane conductance regulator activation by the solvent ethanol: implications for topical drug delivery" @default.
• W2182177768 cites W1509342485 @default.
• W2182177768 cites W1970958133 @default.
• W2182177768 cites W2004978232 @default.
• W2182177768 cites W2005417070 @default.
• W2182177768 cites W2016941225 @default.
• W2182177768 cites W2017954581 @default.
• W2182177768 cites W2022824867 @default.
• W2182177768 cites W2026718479 @default.
• W2182177768 cites W2038048838 @default.
• W2182177768 cites W2039056646 @default.
• W2182177768 cites W2047198892 @default.
• W2182177768 cites W2052436780 @default.
• W2182177768 cites W2054989342 @default.
• W2182177768 cites W2064883859 @default.
• W2182177768 cites W2068523982 @default.
• W2182177768 cites W2072334346 @default.
• W2182177768 cites W2077732883 @default.
• W2182177768 cites W2080212245 @default.
• W2182177768 cites W2080648107 @default.
• W2182177768 cites W2112530308 @default.
• W2182177768 cites W2115806629 @default.
• W2182177768 cites W2128270521 @default.
• W2182177768 cites W2131284004 @default.
• W2182177768 cites W2137075043 @default.
• W2182177768 cites W2148114189 @default.
• W2182177768 cites W2148766169 @default.
• W2182177768 cites W2160405912 @default.
• W2182177768 cites W2233150443 @default.
• W2182177768 cites W2339348082 @default.
• W2182177768 doi "https://doi.org/10.1002/alr.21638" @default.
• W2182177768 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4871113" @default.
• W2182177768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26869199" @default.
• W2182177768 hasPublicationYear "2015" @default.
• W2182177768 type Work @default.
• W2182177768 sameAs 2182177768 @default.
• W2182177768 citedByCount "15" @default.
• W2182177768 countsByYear W21821777682017 @default.
• W2182177768 countsByYear W21821777682018 @default.
• W2182177768 countsByYear W21821777682019 @default.
• W2182177768 countsByYear W21821777682020 @default.
• W2182177768 countsByYear W21821777682021 @default.
• W2182177768 crossrefType "journal-article" @default.
• W2182177768 hasAuthorship W2182177768A5026337502 @default.
• W2182177768 hasAuthorship W2182177768A5035736153 @default.
• W2182177768 hasAuthorship W2182177768A5036733807 @default.
• W2182177768 hasAuthorship W2182177768A5050704941 @default.
• W2182177768 hasAuthorship W2182177768A5050727273 @default.
• W2182177768 hasAuthorship W2182177768A5059406657 @default.
• W2182177768 hasAuthorship W2182177768A5061587101 @default.
• W2182177768 hasBestOaLocation W21821777682 @default.
• W2182177768 hasConcept C114682108 @default.
• W2182177768 hasConcept C126322002 @default.
• W2182177768 hasConcept C134018914 @default.
• W2182177768 hasConcept C185592680 @default.
• W2182177768 hasConcept C18903297 @default.
• W2182177768 hasConcept C24998067 @default.
• W2182177768 hasConcept C2776938444 @default.
• W2182177768 hasConcept C2777312293 @default.
• W2182177768 hasConcept C2777714996 @default.
• W2182177768 hasConcept C2778428886 @default.
• W2182177768 hasConcept C2779932011 @default.
• W2182177768 hasConcept C2780161600 @default.
• W2182177768 hasConcept C28938303 @default.
• W2182177768 hasConcept C41625074 @default.
• W2182177768 hasConcept C49039625 @default.
• W2182177768 hasConcept C534529494 @default.
• W2182177768 hasConcept C55493867 @default.
• W2182177768 hasConcept C71924100 @default.
• W2182177768 hasConcept C86803240 @default.
• W2182177768 hasConcept C87568996 @default.
• W2182177768 hasConcept C98274493 @default.
• W2182177768 hasConcept C99975950 @default.
• W2182177768 hasConceptScore W2182177768C114682108 @default.
• W2182177768 hasConceptScore W2182177768C126322002 @default.
• W2182177768 hasConceptScore W2182177768C134018914 @default.
• W2182177768 hasConceptScore W2182177768C185592680 @default.
• W2182177768 hasConceptScore W2182177768C18903297 @default.
• W2182177768 hasConceptScore W2182177768C24998067 @default.
• W2182177768 hasConceptScore W2182177768C2776938444 @default.
• W2182177768 hasConceptScore W2182177768C2777312293 @default.
• W2182177768 hasConceptScore W2182177768C2777714996 @default.
• W2182177768 hasConceptScore W2182177768C2778428886 @default.
• W2182177768 hasConceptScore W2182177768C2779932011 @default.
• W2182177768 hasConceptScore W2182177768C2780161600 @default.
• W2182177768 hasConceptScore W2182177768C28938303 @default.
• W2182177768 hasConceptScore W2182177768C41625074 @default.

• next