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• W2182198751 abstract "The analogy of malignant rat insulinoma cells RIN-5F with human cervical carcinoma cells HeLa, both containing additional copy of tumor-suppressor gene SCGN and HACE1, respectively, was investigated. This necessity was connected with the observed in previous our studies lower phagocyte differentiation potential in vitro on the influence of both cell types, containing such a copy, in comparison with non-containing additional tumor-suppressor gene malignant cells. Similar analogy has been demonstrated in previous our investigations with normal mouse embryonic stem cells (mESCs), containing and non-containing additionally-inserted oncogene copy, with immortalized and non-immortalized normal human trophoblasts. For identification and quantification of proteins, able to connect specifically or non-specifically with peptide SCGN, protein material from E. coli bacteria strains, containing copy of gene for this peptide, inserted by transfection, was isolated and incubated in protein lysates from rat brain and pancreas, known as organs with the most active expression of this peptide. The so prepared protein fractions were subjected on label-free tandem massspectrometry (LC-MS/MS) assay with subsequent computer processing. Suggested in our previous studies abilities of protein SCGN to connect with cytoskeleton elements were confirmed. On this base, a mechanism for indirect influence of this peptide on the control of cell growth and proliferation, has been proposed. Future studies are necessary in this direction." @default.
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• W2182198751 date "2012-01-06" @default.
• W2182198751 modified "2023-09-23" @default.
• W2182198751 title "Experimental rodent and human models for balanced activity of oncogenes and tumor-suppressor genes and their protein products" @default.
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