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- W2182649061 abstract "The effect of natural and synthetic inhibitors on α-chymotrypsin activity has been studied under in vitro conditions. The chromogenic substrate Suc-(Gly) 2 -Phe-NAn (20 mmol . 0.1 ml -1 ) and Tris/HCl buffer (0.7 ml) was incubated at 37 °C for 15 minutes together with other components. In the first experimental series, the natural inhibitors, hydrolysable tannin, Tanifarm, Farmatan and Pycnogenol at concentrations of 18.75, 37.5 and 75 µg.0.1 ml -1 were added. The same concentrations of synthetic inhibitors I 1 (Ala 2- Leu-NH-EtPh), I 2 (Suc-(Ala) 2- Pro-NH-EtPh), and I 3 (Glt-(Ala) 2- Pro-NH-EtPh) were added in the second series of experiments. The reaction was initiated by α-chymotrypsin (200 µg . 0.1 ml -1 ). A significant decrease (P ≤ 0.001) activity of α-chymotrypsin was found after the addition of natural and synthetic inhibitors in comparison to the control group. The activity of α-chymotrypsin was in order: Pycnogenol < Tanifarm < Tannin < Farmatan after the addition of natural inhibitors, and I 3 < I 1 < I 2 after addition of synthetic inhibitors. The lowest activity of chymotrypsin was recorded after the addition of Pycnogenol (2.88 ηkat.l -1 ) and a synthetic inhibitor I 3 (3.57 ηkat.l -1 ). Our experiment has the potential to contribute useful information regarding the activity of chymotrypsin after the addition of various natural and synthetic inhibitors." @default.
- W2182649061 created "2016-06-24" @default.
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- W2182649061 date "2006-01-01" @default.
- W2182649061 modified "2023-09-27" @default.
- W2182649061 title "ACTIVITY OF α-CHYMOTRYPSIN AFTER ADDITION OF NATURAL AND SYNTHETIC INHIBITORS" @default.
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