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• W2182709503 abstract "The gene CALl (also known as CDC43) of Saccharomyces cerevisiae encodes the fl subunit of geranylgeranyl transferase I (GGTase I), which modifies several small GTPases. Biochemical analyses of the mutant enzymes encoded by call-l, and cdc43-2 to cdc43-7, expressed in bacteria, have shown that all of the mutant enzymes possess reduced activity, and that none shows temperature-sensitive enzymatic activities. Nonetheless, all of the calt/cdc43 mutants show tem- perature-sensitive growth phenotypes. Increase in sol- uble pools of the small GTPases was observed in the yeast mutant cells at the restrictive temperature in vivo, suggesting that the yeast prenylation pathway itself is temperature sensitive. The call-1 mutation, located most proximal to the C-terminus of the protein, differs from the other cdc43 mutations in several respects. An increase in soluble Rholp was observed in the call-1 strain grown at the restrictive temperature. The temperature-sensitive phenotype of call-1 is most" @default.
• W2182709503 created "2016-06-24" @default.
• W2182709503 creator A5034973819 @default.
• W2182709503 date "1996-01-01" @default.
• W2182709503 modified "2023-09-27" @default.
• W2182709503 title "Mutational analysis of the p-subunit of yeast geranylgeranyi transferase !" @default.
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