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- W2182738052 abstract "Theefficiency ofa Millitube MF cartridge filter, a membrane filter, for recoveryofpoliovirus from100-gal volumes ofbothfresh (tap) andestuarine waterwas determined. Inthehighmultiplicity ofvirus input-output experiments, recoveryof97%orgreater ofinput virus was achieved inbothtypesof waterwhenthefinal concentration ofdivalent cation asMg2+was 1,200Pg/ml andthepH was 4.5. Virus was effectively eluted fromthemembrane cartridge with5xnutrient broth in0.05 M carbonate-bicarbonate buffer atpH 9.0. Four elutions of250mleachwereused. Inthelowmultiplicity ofvirus input-output experiments underthesame cationic andpH conditions, up to67%ofthe inputvirus was recovered whenthevirus was further concentrated fromthe eluates bytheaqueouspolymer two-phase separation technique. Thevolume reduction was 126,000-190,000 to1.Theuseofthecombined techniques, i.e., membraneadsorption followed byaqueouspolymer two-phase separation, provided a highly sensitive, simple, andremarkably reliable sequential methodology forthequantitative recoveryofpoliovirus occurring atmultiplicities as lowas1to2plaque-forming units per5galofwater. Theoccurrence anddistribution ofviruses in waterareofmajorconcern toenvironmental health officials. Theenigmaofthevirus-inwaterproblem isdirectly related tothetechnical deficit ofareliable method forrecovering" @default.
- W2182738052 created "2016-06-24" @default.
- W2182738052 creator A5000790303 @default.
- W2182738052 date "1972-01-01" @default.
- W2182738052 modified "2023-09-27" @default.
- W2182738052 title "VirusinWater II.Evaluation ofMembrane Cartridge Filters forRecovering Low Multiplicities ofPoliovirus fromWater" @default.
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- W2182738052 hasPublicationYear "1972" @default.
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