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- W2182752193 abstract "A simple, fast, and precise reverse phase, isocratic HPLC method was developed for the separation and quantification of olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide in bulk drug and pharmaceutical dosage form. The quantification was carried out using thermo hypersil ODS-C18 (250 mm × 4.6 mm, 5.0 µ) column and mobile phase comprised of methanol: 0.05M potassium dihydrogen phosphate with triethylamine (80:20 v/v) pH 3 adjusted with orthophosphoric acid. The flow rate was 0.8 ml/min and the effluent was monitored at 230 nm. The retention time of olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide were 5.25, 4.55 and 3.31 min respectively. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of detection and limit of quantitation. Linearity of olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide were in the range of 20 to 60 mg/ml, 5 to 25 mg/ml and 2.5 to 12.5 mg/ml respectively. The percentage recoveries of all the drugs were 99.46%, 99.92% and 100.01% for olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide respectively from the tablet formulation. The proposed method is suitable for simultaneous determination of olmesartan medoxomil, amlodipine besylate and hydrochlorothiazide in pharmaceutical dosage form and bulk drug." @default.
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- W2182752193 date "2011-01-01" @default.
- W2182752193 modified "2023-09-24" @default.
- W2182752193 title "Simultaneous Quantitation of Olmesartan medoxomil, Amlodipine besylate and Hydrochlorothiazide in Pharmaceutical dosage form by using HPLC" @default.
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