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- W2182775488 abstract "The present investigation is the first work on in vitro propagation of an elite endangered date palm (Phoenix dactylifera L.) Ghazal cultivar, through somatic embryogenesis. This cultivar is grown in Siwa Oasis, which is suffering an excessive rise in the subsoil water levels. Consequently, the fertile soils are subjected to deterioration and sanitization. This study was conducted to determine the effect of various concentrations of folic acid (vitamin B9) and biotin (vitamin H), on callus initiation, embryogenic callus formation and embryos number of date palm Ghazal cv. Shoot tip explants were cultured on modified Murashige and Skoog (MS) medium supplemented with 100 mg/l of 2,4-dichlorophenoxy acetic acid (2,4-D), 3 mg/l isopentenyl adenine (2iP) and vitamins (B9 and H) at 0.0, 0.1, 0.5 and 1 mg/l for each or in combinations. The best medium for callus initiation and formation was modified MS medium supplemented with vitamin B9 at 0.1 mg/l and 0.5 mg/l vitamin H. The highest percentage of embryogenic callus formation was obtained on modified MS medium, supplemented with 10 mg/l 2, 4-D, 3 mg/l 2iP, 0.5 mg/l vitamin B9 and 1 mg/l vitamin H. This treatment also recorded increase in the embryogenic callus fresh weight. Moreover, the maximum number of embryos was formed at modified MS medium supplemented with 0.1 mg/l NAA, 0.5 mg/l vitamin B9 and 1 mg/l vitamin H, compared control treatment. Organic substances seem to be a limiting factor in in vitro date palm culture and their addition as vitamins (B9 and H) have a beneficial effect." @default.
- W2182775488 created "2016-06-24" @default.
- W2182775488 creator A5054500461 @default.
- W2182775488 date "2015-01-01" @default.
- W2182775488 modified "2023-09-27" @default.
- W2182775488 title "In vitro Propagation of the Endangered Date Palm (Phoenix dactylifera L.) Ghazal cv. 1-Effect of Folic Acid and Biotin on Callus Formation and Differentiation" @default.
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