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- W218296870 abstract "In a previous study, we have demonstrated that microspectrophotometry coupled with a digital image processing is able to determine fractional binding of oxygen to myoglobin in an individual single cardiac myocyte isolated from the ventricles of the rat (Takahashi and Doi, 1995). Using this technique, we have determined the oxygen pressure (Po2) gradients from the extracellular space to cytosol in a quiescent cardiomyocyte (Takahashi and Doi, 1996). We also found that the Po2 gradients diminish as the cell becomes hypoxic (Takahashi and Doi, 1996). This reduction of Po2 gradients may partially restore the oxygenation of the intracellular space so that oxygen supply to mitochondria required for minimal electron flux through the respiratory chain is maintained even in severe hypoxia. We, however, were not able to quantitate the relative contributions of the extracellular (from extracellular medium to sarcolemma) and intracellular (from the sarcolemma to cytosol) Po2 gradients in the control of intracellular Po2. Thus, we undertook the present study to directly assess the magnitude of intracellular Po2 gradients with a subcellular spatial resolution in the single isolated cardiomyocyte." @default.
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- W218296870 date "1998-01-01" @default.
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- W218296870 title "Direct Estimation of Intracellular Po2 Gradients in a Single Cardiomyocyte of the Rat" @default.
- W218296870 cites W1504836130 @default.
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- W218296870 doi "https://doi.org/10.1007/978-1-4615-4863-8_49" @default.
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