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- W2182977237 abstract "OLIVEIRA, Fernanda Arboite de Characterization of Salmonella Enteritidis isolated from foods and human samples based on antibiotic susceptibility testing, PCR-ribotyping, and Pulsed-Field Gel Electrophoresis (PFGE). 2009. 115f. Tese (Doutorado) – Programa de Pos-Graduacao em Ciencia e Tecnologia Agroindustrial. Universidade Federal de Pelotas, Salmonella is one of the principal agents of food-borne diseases transmitted all over the world and in the State of Rio Grande do Sul, Southern Brazil, this microorganism has been identified as the principal agent of food-borne outbreaks in the last years. Central Laboratory of RS has sampled many isolates of S. Enteritidis from foods involved in outbreaks and from human origin, however there is no study comparing food samples with human samples. This study aimed to characterize Salmonella enterica subsp. enterica (S.) serovar Enteritidis isolated from food (n=152), blood (n=12) and feces (n=68) and investigate the clonal relationship among S. Enteritidis involved in foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil. The isolates were submitted to antimicrobial susceptibility testing, PCRribotyping, and XbaI macrorestriction Pulsed Field Gel Eletrophoresis (PFGE). To confirm the clonal relationship, a PCR-ribotyping amplicon was sequenced in 26 randomly chosen S. Enteritidis typed. The results indicate that a strain S. Enteritidis or a very closely related clone of this microorganism was involved in salmonelosis outbreaks occurred in RS. To confirm the clonal relationship, a PCR-ribotyping amplicon was sequenced in 26 randomly chosen S. Enteritidis isolated from foods typed. High rates of ampicilin and nalidixic acid resistance were found. Strains isolated from foods and humans sources collected in different geographic regions of RS clustered together and presents a common resistance profile. All strains demonstrated similar PCR-ribotyping patterns, being that the predominant profile R1 grouped 47.5% of the human strains (blood and feces) and 94.7% of food strains. One PFGE profile (P1) was found in the majority of the food strains (92.8%) and humans strains (96.25%). The results indicate that a specific strain of S. Enteritidis was isolated from foods and humans involved in salmonellosis outbreaks occurred in Southern Brazil. Key-words: Salmonella Enteritidis, PFGE, PCR-ribotyping, antimicrobial resistance, salmonelosis, foodborne disease" @default.
- W2182977237 created "2016-06-24" @default.
- W2182977237 creator A5091814673 @default.
- W2182977237 date "2009-01-01" @default.
- W2182977237 modified "2023-09-27" @default.
- W2182977237 title "CARACTERIZAÇÃO DE Salmonella Enteritidis ISOLADAS DE ALIMENTOS E DE HUMANOS POR SUSCEPTIBILIDADE A ANTIMICROBIANOS, PCR- RIBOTIPIFICAÇÃO E ELETROFORESE DE CAMPO PULSADO (PFGE)" @default.
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