Matches in SemOpenAlex for { <https://semopenalex.org/work/W2183103678> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2183103678 abstract "Alkaline protease from a mutant of Bacillus licheniformis Bl8 was purified from the culture supernatant by employing the methods such as ammonium sulphate precipitation, DEAE cellulose chromatography followed by Gel filtration using Sephadex G-100. The yield of the enzyme after purification was found to be 10%. Protease was found to be homogenous when examined by SDS-PAGE and the enzyme showed that it has a molecular weight of 28 KDa. Characterization studies were carried out using the purified enzyme. While the optimum pH and temperature for the activity of alkaline protease was found to be 10 and 50°C and stable in the pH range 5.0 - 12.0. The thermo stability exhibited by protease ranged from 30-70°C. Among various protease inhibitors PMSF strongly inhibited the enzyme activity revealing that the enzyme in the present study is serine alkaline protease. Ca and Mn had a slight enhancing effect on the activity of the 2+ +2 enzyme. High level of hydrolytic activity was shown by casein and also found that purified alkaline protease digested the human blood clot, coagulated white egg to soluble form and also digested chicken skin upon the prolonged incubation of enzyme with chicken skin. The protease showed good compatibility and stability in the presence of CaCl and glycine with Nirma, Surf excel and Wheel. The enzyme retained 20-40% activity with 2 most of the detergents tested even after 3hrs. The supplementation of the enzyme preparation in detergent completely removed the blood stain of the cloth. The enzyme followed a typical Michaelis-Menten kinetics. Apparent Km value was found to be 3.2mgml . 1" @default.
- W2183103678 created "2016-06-24" @default.
- W2183103678 creator A5088379711 @default.
- W2183103678 date "2015-01-01" @default.
- W2183103678 modified "2023-10-01" @default.
- W2183103678 title "Purification and Characterization of Alkaline Protease from a Mutant Bacillus licheniformis Bl8" @default.
- W2183103678 cites W1572955611 @default.
- W2183103678 cites W2151351207 @default.
- W2183103678 hasPublicationYear "2015" @default.
- W2183103678 type Work @default.
- W2183103678 sameAs 2183103678 @default.
- W2183103678 citedByCount "2" @default.
- W2183103678 countsByYear W21831036782016 @default.
- W2183103678 countsByYear W21831036782017 @default.
- W2183103678 crossrefType "journal-article" @default.
- W2183103678 hasAuthorship W2183103678A5088379711 @default.
- W2183103678 hasConcept C163994747 @default.
- W2183103678 hasConcept C181199279 @default.
- W2183103678 hasConcept C185592680 @default.
- W2183103678 hasConcept C193363816 @default.
- W2183103678 hasConcept C2776142715 @default.
- W2183103678 hasConcept C2776428949 @default.
- W2183103678 hasConcept C2776527165 @default.
- W2183103678 hasConcept C2776714187 @default.
- W2183103678 hasConcept C2777272437 @default.
- W2183103678 hasConcept C2777807008 @default.
- W2183103678 hasConcept C2780865716 @default.
- W2183103678 hasConcept C28212737 @default.
- W2183103678 hasConcept C43617362 @default.
- W2183103678 hasConcept C523546767 @default.
- W2183103678 hasConcept C54355233 @default.
- W2183103678 hasConcept C55493867 @default.
- W2183103678 hasConcept C86803240 @default.
- W2183103678 hasConceptScore W2183103678C163994747 @default.
- W2183103678 hasConceptScore W2183103678C181199279 @default.
- W2183103678 hasConceptScore W2183103678C185592680 @default.
- W2183103678 hasConceptScore W2183103678C193363816 @default.
- W2183103678 hasConceptScore W2183103678C2776142715 @default.
- W2183103678 hasConceptScore W2183103678C2776428949 @default.
- W2183103678 hasConceptScore W2183103678C2776527165 @default.
- W2183103678 hasConceptScore W2183103678C2776714187 @default.
- W2183103678 hasConceptScore W2183103678C2777272437 @default.
- W2183103678 hasConceptScore W2183103678C2777807008 @default.
- W2183103678 hasConceptScore W2183103678C2780865716 @default.
- W2183103678 hasConceptScore W2183103678C28212737 @default.
- W2183103678 hasConceptScore W2183103678C43617362 @default.
- W2183103678 hasConceptScore W2183103678C523546767 @default.
- W2183103678 hasConceptScore W2183103678C54355233 @default.
- W2183103678 hasConceptScore W2183103678C55493867 @default.
- W2183103678 hasConceptScore W2183103678C86803240 @default.
- W2183103678 hasLocation W21831036781 @default.
- W2183103678 hasOpenAccess W2183103678 @default.
- W2183103678 hasPrimaryLocation W21831036781 @default.
- W2183103678 hasRelatedWork W2115963188 @default.
- W2183103678 hasRelatedWork W2119132545 @default.
- W2183103678 hasRelatedWork W2137986600 @default.
- W2183103678 hasRelatedWork W2168457578 @default.
- W2183103678 hasRelatedWork W2184097839 @default.
- W2183103678 hasRelatedWork W2184387312 @default.
- W2183103678 hasRelatedWork W2185796327 @default.
- W2183103678 hasRelatedWork W2373216997 @default.
- W2183103678 hasRelatedWork W2379643863 @default.
- W2183103678 hasRelatedWork W2394673961 @default.
- W2183103678 hasRelatedWork W2396982275 @default.
- W2183103678 hasRelatedWork W2408533025 @default.
- W2183103678 hasRelatedWork W2551414021 @default.
- W2183103678 hasRelatedWork W2751295724 @default.
- W2183103678 hasRelatedWork W2793041491 @default.
- W2183103678 hasRelatedWork W2894954741 @default.
- W2183103678 hasRelatedWork W2953027156 @default.
- W2183103678 hasRelatedWork W2185781905 @default.
- W2183103678 hasRelatedWork W2187437158 @default.
- W2183103678 hasRelatedWork W2316798757 @default.
- W2183103678 isParatext "false" @default.
- W2183103678 isRetracted "false" @default.
- W2183103678 magId "2183103678" @default.
- W2183103678 workType "article" @default.