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- W2183176453 abstract "Two high ethanol-yielding recombinants of Saccharomyces cerevisiae were made by protoplast fusion. The parental strains were isolated from 25 day old palm wines (Raphia raphia and Elaensis guineensis) obtained from South-eastern Nigeria. The parental strains were isolated on Glucose Yeast Agar at 28 oC. They had ethanol tolerance in the range of 18% – 20% v/v ethanol. Cassava starch hydrolysates produced by both acid and enzyme hydrolysis methods were used as substrates for fermentation to ethanol by the isolates and recombinants. The enzyme hydrolysates enabled production of higher ethanol levels (13.7% v/v max) by the isolates than the acid hydrolysates (8.7% v/v max). Twenty six randomly selected regenerate recombinants were examined after protoplast fusion for the desired markers which were enhanced ethanol tolerance and production. Only two of the recombinants showed the desired recombination. They exhibited an enhanced tolerance of 24% v/v ethanol for designate F5 and F14. And also gave higher yields of ethanol (16% v/v). One recombinant F16 performed significantly lower than the parental strains yielding only 4.6% v/v ethanol. [Life Science Journal. 2008; 5(4): 64 – 68] (ISSN: 1097 – 8135)." @default.
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- W2183176453 date "2008-01-01" @default.
- W2183176453 modified "2023-09-27" @default.
- W2183176453 title "Production of high-ethanol-yielding Saccharomyces cerevisiae of palm wine origin by protoplast fusion" @default.
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