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- W2183177737 abstract "Theindependent repair ofmismatched nucleotides present inheteroduplex DNA hasbeenusedtoexplain geneconversion andmap expansion after general genetic recombination. We haveconstructed andpurified heteroduplex plasmid DNAsthatcontain heteroallelic 10-base-pair insertion-deletion mismatches. TheseDNA substrates are similar instructure totheheteroduplex DNA intermediates thathavebeenproposed tobe produced during thegenetic recombination ofplasmids. TheseDNA substrates were transformed into wild-type andmutantEscherichia coli strains, andthefate oftheheteroduplex DNA was determined byboth restriction mapping andgenetic tests. Independent repair events thatyielded a wild-type Tetr gene were observed atafrequency ofapproximately 1%inbothwild-type andrecBrecCsbcBmutantE.coli strains. The independent repair ofsmall insertion-deletion-type mismatches separated by1,243 basepairs was found tobe reduced byrecF, recJ,andssbsingle mutations inanotherwise wild-type genetic background andreduced by recF, recJ,andrecOmutations inarecBrecCsbcBgenetic background (the ssbmutation was nottested inthe latter background). Independent repair ofsmall insertion-deletion-type mismatched nucleotides that were as close as312nucleotides apart was observed. There was no apparent biasinfavor oftheinsertion ordeletion ofmutantsequences." @default.
- W2183177737 created "2016-06-24" @default.
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- W2183177737 date "1989-01-01" @default.
- W2183177737 modified "2023-09-24" @default.
- W2183177737 title "GeneConversion inEscherichia coli: theRecFPathway for Resolution ofHeteroduplex DNA" @default.
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