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- W2183299897 abstract "A simple, precise and reproducible reverse phase, i socratic high performance liquid chromatographic method was developed and validated for the quantitative determination of Vancomycin in blood plasma. The quantification was carried out using a HPLC system consisted of a Jasco UV- 975 intelligent UV visible detector, Jasco; PU-980 intelligent HPLC pump, Hypersil BDS C18 ; 150 mm x 4.6 mm , Clarity lite data apex 2003 software, REMI centrifuge machine (electrometer corporation);Model-R8C, with a mobile phase consisting of 10% acetonitrile in a 25 mmol/L or 50 mmol/L phosphate buffer in pH 7.0 and pH 3.2, at a flow rate of 1.0 and 1.5 ml/min ,detection wavelength were us ed at 229 and 270 nm. The method was validated and developed for selection of suitable I nternal Standards, linearity, accuracy, robustness and solution stability. The linearity of the proposed method was investigated in the range of 5-100 �g /ml, R 2 = 0.995 and 0.997 for reference standard vancomyci n solution and in blood serum without any internal standard respectiv ely and 0.998 with ondanosatrone when it was used as a I.S. The solution stability of vancom ycin was studied at pH 7.0 and pH 3.2 under the same ionic strength condition at 37 o C. A stability indicating HPLC method specific for vancomycin was developed and validated for use in t he research. The proposed method was developed to apply for the analysis of vancomycin i n bulk forms and also, the method was extended for determination of various pharmacokinetic parameters of vancomycin from in vivo blood plasma serum." @default.
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- W2183299897 date "2010-01-01" @default.
- W2183299897 modified "2023-09-23" @default.
- W2183299897 title "Development of method of analysis for estimating the Vancomycin in blood plasma by RP-HPLC method: Application to in vivo Studies" @default.
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