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• W2183454865 abstract "A moderately halophilic bacterium, Bacillus sp. HS-4 was isolated from soil samples collected from Khewra salt range (Pakistan). Experiments were set to observe the effects of different pH, temperature, incubation time, inoculum's size, salt concentration (NaCl), agitation rate, carbon and nitrogen sources and metal ions on the growth of HS-4 and its protease activity. Optimum biomass and proteolytic activity was achieved after 24 hrs of incubation, at 37°C, pH 8 with 8 % salinity and 150 rpm agitation (295 U/ml). Among the various nitrogen sources investigated, different low cost agricultural byproducts in the form of defatted soybean meal was found to be the best inducer of alkaline protease, while inorganic nitrogen sources in the form of ammonium salts (NH Cl and (NH ) SO )showed reduced bacterial growth and enzyme activity. Among 4 42 4 the carbon sources tested, rice bran maximized the enzyme production. An increase in protease activity was observed in the presence of Ca and Mg ions while protease activity was significantly reduced by Cu and 2+ 2+ 2+" @default.
• W2183454865 created "2016-06-24" @default.
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• W2183454865 date "2011-01-01" @default.
• W2183454865 modified "2023-09-23" @default.
• W2183454865 title "Extracellular Alkaline Protease by a Newly Isolated Halophilic Bacillus sp" @default.
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