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- W2183558612 abstract "Bacopa monniera commonly known in India as 'Brahmi', it is an important ancient ayurvedic medicinal plant. In the traditional system of medicine Brahmi is used as a nervine tonic. It is also used to treat asthma, epilepsy, enlargement of spleen, rheumatism. It possesses anti-inflammatory, analgesic and antipyretic activity. It contains several alkaloids e.g., nicotine, brahmine, herpestine and saponins such as bacosides A,B,C and D. Propagation of B. monniera through seeds is slow due to short viability and frequent seedling death. Its vegetative propagation is slow due to poor performance of propagules. Therefore, development of a rapid clonal multiplication of this medicinally important herb has become imperative in order to reduce the pressure on natural populations and constant supply of plant materials for pharmaceutical industries. The morphogenic potential of node, internode and leaf explants was investigated to develop reliable protocol for shoot regeneration. A range of cytokinins tested (6-benzylaminio purinre, BAP; kinetin, KIN; thidiazuron, TDZ; and 2-isopenteny adenine,2-iP) for multiple shoot induction. Of the four cytokinins tested TDZ(6.8µM) and BAP(8.9µM) proved superior to other treatments. A mass in vitro propagation system devoid of growth regulators has been developed. MS medium supplemented with an antibiotic (trimethoprim) or a fungicide (bavistin) supports direct shoot bud induction on internode and leaf explants. Bavistin showed a marked cytokinin like activity thus optimum adventitious shoot buds induction occurred on MS medium supplemented with 1569.14µM bavistin. MS medium supplemented with 0.44µM BAP and 1.14µM IAA found most suitable for shoot elongation. In vitro derived shoots exhibited better rooting response on MS medium containing 4.9µM IBA. Regenerated rooted plantlets of B. monniera were successfully acclimatized in soilrite. The in vitro raised plants were grown normally in the field without showing any morphological variation." @default.
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- W2183558612 date "2010-01-01" @default.
- W2183558612 modified "2023-09-27" @default.
- W2183558612 title "Effective organogenesis from different explants of Bacopa monniera L. (Wettst.) - an important medicinal plant." @default.
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