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• W2183611088 abstract "The terminator tI, located approximately 280 nucleotides beyond the int gene of bacteri o- phage lambda, is considered an intrinsic terminator due to its GC-rich region of dyad symmetry followed by a T stretch. In order to study the effect of tI structure in T3 RNA polymerase-mediated transcription termination, three different point mutations tI1, tI2 and tI3 were analyzed. DNA fragments containing the tI mutations were cloned into the pBS+ vector for in vitro transcription studies. All the tI mutations map- ping in the tI stem-loop structure decrease the in vitro transcription termination of tI from 85% for the wild type terminator to 46-60% using the T3 RNA polymerase." @default.
• W2183611088 created "2016-06-24" @default.
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• W2183611088 date "2000-01-01" @default.
• W2183611088 modified "2023-10-18" @default.
• W2183611088 title "Effect of λ λ tI Point Mutations on T3 RNA Polymerase-Mediated Transcription Termination" @default.
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