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- W2183611245 abstract "Weexpressed thegagandproteinase regions ofhumanimmunodeficiency virus (HIV)type1bytranscription andtranslation invitro. A synthetic RNA spanning thegag andprodomains gaveprimarily theunprocessed capsid precursorpr53. Efficient cleavage ofthis precursorwas observed whenthegag andpro domains were placed inthesame translational reading frame, yielding equimolar amountsofthegag protein andof proteinase (PR). Expression ofHIVtype1PRinEscherichia coli asa fusion protein gaverapid autocatalytic processing toan HIV-specific protein ofapproximately 11kilodaltons. HIVPRgenerated inE.coli specifically induced cleavage oftheHIVcapsid precursor,whereas deletion ofthecarboxy-terminal 17aminoacids ofthe proteinase rendered itinactive. Inhibitor studies showed that theenzyme was insensitive toinhibitors ofserine andcysteine proteinases andmetalloproteinases andwas inhibited onlybya veryhighconcentration (1mM) ofpepstatin A. Thegenomesofallreplication-competent retroviruses," @default.
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- W2183611245 date "1988-01-01" @default.
- W2183611245 modified "2023-10-16" @default.
- W2183611245 title "gag Precursor Proteins of HumanImmunodeficiency Virus(HIV)Type1 byHIV Proteinase Generated inEscherichia coli" @default.
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