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- W2183611538 abstract "Proteases are well known enzymes for their wide range application in food industry, detergent industry and pharmaceuticals industry for the preparation of ointments and medicine. They are also widely used in leather industry for dehairing and bating of hides as an alternative for toxic chemicals which in turn hamper the environment. As enzymes have proved to solve unresolved technical issues relating to pollution control the present study was undertaken to screen for organisms which could produce extracellular proteases. Organisms were isolated from tannery effluent, in which 13 isolates were obtained and among which 5 isolates were found to produce extracellular proteases. Biochemical test revealed the organisms to be Aeronomas sp., Alcaligenes sp., Bacillus sp., Staphylococcus sp. and Pseudomonas sp. The stability of the protease was also studied under temperature and pH. The organism was found to grow over a wide range of pH and temperatures. Among the 5 isolates Aeronomas sp., Bacillus sp., Staphylococcus sp. and Pseudomonas sp. showed enzyme production at pH 8.0 and Alcaligenes sp at pH 9.0. The enzyme production for all the isolates was found to be between 45°C to 65°C with ane optimum temperature of 55°C. Zymography analysis in this present study showed extracellular caseinase activity by substrate hydrolysis." @default.
- W2183611538 created "2016-06-24" @default.
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- W2183611538 date "2011-01-01" @default.
- W2183611538 modified "2023-09-26" @default.
- W2183611538 title "A COMPARATIVE ANALYSIS OF PROTEASE PRODUCING MICROBES ISOLATED FROM TANNERY EFFLUENT" @default.
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