Matches in SemOpenAlex for { <https://semopenalex.org/work/W2183618520> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2183618520 endingPage "435" @default.
- W2183618520 startingPage "429" @default.
- W2183618520 abstract "Flavonoid 3’5’-hydroxylase (F3’5’H), a member of the cytochrome P450 family, is the key enzyme for the expression of blue or purple flower color. By a polymerase chain reaction (PCR)-based strategy using a degenerate primer designed from the conservative region of the F3’5’H genes, a full-length cDNA (accession number AB127340) was cloned from blue flower tepals of Muscari armeniacum ‘Blue Pearl’, and its genomic clone designated MaP450 (accession number AB127341), was isolated from leaves. Nucleotide sequence analysis revealed that MaP450 contains an open reading frame (ORF) of 1512 bp, which consists of two exons (888 and 624 bp) encoding a polypeptide of 503 amino acid residues. The deduced amino acid sequence of MaP450 has several conserved regions of the cytochrome P450 proteins, but shows only 34-37% identities with those of previously reported F3’5’H genes. Southern blot analysis showed that there are 5-6 copies of MaP450 in the genome of M. armeniacum ‘Blue Pearl’. High-performance liquid chromatography (HPLC) and reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that MaP450 is expressed developmentally paralleling anthocyanidin accumulation in the tepal. The expression pattern of MaP450 is similar to those of the majority of genes involved in the flavonoid biosynthetic pathway. Transcripts of MaP450 were markedly detected in tepals of Muscari genotypes with colored flowers, but only slightly detected in those of genotypes with white flowers. These results indicate that MaP450 may be a P450 gene involved in the flavonoid biosynthetic pathway in Muscari species." @default.
- W2183618520 created "2016-06-24" @default.
- W2183618520 creator A5005824874 @default.
- W2183618520 creator A5022942832 @default.
- W2183618520 creator A5037163550 @default.
- W2183618520 creator A5072323000 @default.
- W2183618520 creator A5082247878 @default.
- W2183618520 date "2005-05-01" @default.
- W2183618520 modified "2023-10-17" @default.
- W2183618520 title "ISOLATION AND CHARACTERIZATION OF A CYTOCHROME P450 GENE FROM MUSCARI ARMENIACUM" @default.
- W2183618520 cites W1985418187 @default.
- W2183618520 cites W1993320561 @default.
- W2183618520 cites W2016193444 @default.
- W2183618520 cites W2050041099 @default.
- W2183618520 cites W2106882534 @default.
- W2183618520 cites W2141358713 @default.
- W2183618520 cites W2144634347 @default.
- W2183618520 cites W2150297520 @default.
- W2183618520 doi "https://doi.org/10.17660/actahortic.2005.673.54" @default.
- W2183618520 hasPublicationYear "2005" @default.
- W2183618520 type Work @default.
- W2183618520 sameAs 2183618520 @default.
- W2183618520 citedByCount "3" @default.
- W2183618520 countsByYear W21836185202016 @default.
- W2183618520 countsByYear W21836185202023 @default.
- W2183618520 crossrefType "journal-article" @default.
- W2183618520 hasAuthorship W2183618520A5005824874 @default.
- W2183618520 hasAuthorship W2183618520A5022942832 @default.
- W2183618520 hasAuthorship W2183618520A5037163550 @default.
- W2183618520 hasAuthorship W2183618520A5072323000 @default.
- W2183618520 hasAuthorship W2183618520A5082247878 @default.
- W2183618520 hasConcept C181199279 @default.
- W2183618520 hasConcept C2775941552 @default.
- W2183618520 hasConcept C526171541 @default.
- W2183618520 hasConcept C55493867 @default.
- W2183618520 hasConcept C59822182 @default.
- W2183618520 hasConcept C60644358 @default.
- W2183618520 hasConcept C78458016 @default.
- W2183618520 hasConcept C86803240 @default.
- W2183618520 hasConceptScore W2183618520C181199279 @default.
- W2183618520 hasConceptScore W2183618520C2775941552 @default.
- W2183618520 hasConceptScore W2183618520C526171541 @default.
- W2183618520 hasConceptScore W2183618520C55493867 @default.
- W2183618520 hasConceptScore W2183618520C59822182 @default.
- W2183618520 hasConceptScore W2183618520C60644358 @default.
- W2183618520 hasConceptScore W2183618520C78458016 @default.
- W2183618520 hasConceptScore W2183618520C86803240 @default.
- W2183618520 hasIssue "673" @default.
- W2183618520 hasLocation W21836185201 @default.
- W2183618520 hasOpenAccess W2183618520 @default.
- W2183618520 hasPrimaryLocation W21836185201 @default.
- W2183618520 hasRelatedWork W1974616187 @default.
- W2183618520 hasRelatedWork W1993279268 @default.
- W2183618520 hasRelatedWork W2062799029 @default.
- W2183618520 hasRelatedWork W2070232054 @default.
- W2183618520 hasRelatedWork W2077456593 @default.
- W2183618520 hasRelatedWork W2089025702 @default.
- W2183618520 hasRelatedWork W2992231355 @default.
- W2183618520 hasRelatedWork W3215139134 @default.
- W2183618520 hasRelatedWork W4234352422 @default.
- W2183618520 hasRelatedWork W4240757957 @default.
- W2183618520 isParatext "false" @default.
- W2183618520 isRetracted "false" @default.
- W2183618520 magId "2183618520" @default.
- W2183618520 workType "article" @default.