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- W2183619311 abstract "Thexylanase gene fromtheruminal bacterium Bacteroides ruminicola 23ishighly expressed incolonic Bacteroides species whencarried on plasmid pVAL-RX.Inordertostabilize xylanase expression intheabsence ofantibiotic selection, thexylanase gene was introduced intothechromosome ofBacteroides thetaiotaomicron 5482byusing suicide vectorpVAL-7.Xylanase activity intheresulting strain, B.thetaiotaomicron BTX,was about30%ofthatobserved inB.thetaiotaomicron 5482containing thexylanase gene on pVAL-RX.Thedata obtained fromcontinuous culture experiments using antibiotic-free mediumshowedthatexpression ofxylanase activity instrain BTX was extremely stable, withno demonstrated lossoftheinserted xylanase gene over 60 generations, withdilution rates from0.42 to0.03h-'.Incontrast, theplasmid-borne xylanase gene was almost completely lost by60generations intheabsence ofantibiotic selection. Incubation ofstrain BTX withoatspelt xylan resulted inthedegradation ofmore than40% ofthexylantosoluble xylooligomers. Thestability of xylanase expression inB.thetaiotaomicron BTX suggests thatthismicroorganism mightbesuitable for" @default.
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- W2183619311 date "1991-01-01" @default.
- W2183619311 modified "2023-09-27" @default.
- W2183619311 title "Introduction oftheBacteroides ruminicola Xylanase Geneinto theBacteroides thetaiotaomicron Chromosomefor Production ofXylanase Activity" @default.
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