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- W2183621368 abstract "Thebinding ofphytochrome toCibacron Blue3GAwasutilized to develop anewaffinity purification procedure forphytochrome. Brushitepurified phytochrome fromrye(Secale cereak c.v.Cougar) wasboundto agarose-immobilized bluedyein0.1molarpotassium phosphate (pH7.8), contaminating proteins washed outwith 0.5molarKCI, andhomogeneous phytochrome eluted with10millimolar flavin mononucleate. Ninety-five percentofthephytochrome applied bound, and60to65%waseluted, giving a25to30%yield forthecomplete one-day procedure. Affinitypurified ryephytochrome wasidentical toconventionally purified phytochromeinitsbehavior onsodium dodecyl sulfate gels, ingelexclusion chromatography, insedimentation insucrose density gradients andinits spectral properties." @default.
- W2183621368 created "2016-06-24" @default.
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- W2183621368 date "1981-01-01" @default.
- W2183621368 modified "2023-09-27" @default.
- W2183621368 title "Purification ofPhytochrome byAffinity Chromatography on Agarose-Immobilized Cibacron Blue3GA" @default.
- W2183621368 hasPublicationYear "1981" @default.
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