Matches in SemOpenAlex for { <https://semopenalex.org/work/W2183630672> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2183630672 endingPage "7" @default.
- W2183630672 startingPage "341" @default.
- W2183630672 abstract "We used a meq-deleted attenuated MDV-I strain GX0101Δmeq as a vector to construct a recombinant virus expressing the exogenous gene NDV-F. The ORF of exogenous gene NDV-F was inserted into the eukaryotic expression vector pcDNA3.1(-). Then, the expression cassette of NDV-F which contains the CMV promoter was amplified. Simultaneously, we amplified the selected gene Kan+ expression cassette and inserted them into the PMD18-T vector. Tandem expression cassettes were amplified using primers containing the 50-bp homologous arm of MDV-US2. The PCR product was electroporated into EL250 host bacteria containing GX0101Δmeq. Then, the Kan+ expression cassette was deleted from the recombinant virus genome using 1% arabinose. The plasmid of the positive clone which the Kan+ expression cassette was deleted was extracted and transfected into CEFs to rescue the recombinant virus. The recombinant virus was injected into chickens to observe its growth and replication. The recombinant virus rMDV-F containing the exogenous gene NDV-F was rescued successfully. The recombinant virus could duplicate and express well in CEFs, and grow and replicate well in chickens. Using GX0101Δmeq as a vector, combined with a recombinant system of Red E/T and FLP/FRT, we constructed a recombinant virus that expressed the exogenous gene NDV-F. This study could lay the foundation for further study of recombinant viruses." @default.
- W2183630672 created "2016-06-24" @default.
- W2183630672 creator A5002849906 @default.
- W2183630672 creator A5022590540 @default.
- W2183630672 creator A5026299352 @default.
- W2183630672 creator A5032056890 @default.
- W2183630672 creator A5038996257 @default.
- W2183630672 creator A5055421311 @default.
- W2183630672 creator A5057676219 @default.
- W2183630672 creator A5060245635 @default.
- W2183630672 creator A5060245983 @default.
- W2183630672 date "2015-07-01" @default.
- W2183630672 modified "2023-10-14" @default.
- W2183630672 title "[Construction of Recombinant Marek's Disease Virus Expressing the NDV-F gene and its Replication in Chickens and in Vitro]." @default.
- W2183630672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26524905" @default.
- W2183630672 hasPublicationYear "2015" @default.
- W2183630672 type Work @default.
- W2183630672 sameAs 2183630672 @default.
- W2183630672 citedByCount "0" @default.
- W2183630672 crossrefType "journal-article" @default.
- W2183630672 hasAuthorship W2183630672A5002849906 @default.
- W2183630672 hasAuthorship W2183630672A5022590540 @default.
- W2183630672 hasAuthorship W2183630672A5026299352 @default.
- W2183630672 hasAuthorship W2183630672A5032056890 @default.
- W2183630672 hasAuthorship W2183630672A5038996257 @default.
- W2183630672 hasAuthorship W2183630672A5055421311 @default.
- W2183630672 hasAuthorship W2183630672A5057676219 @default.
- W2183630672 hasAuthorship W2183630672A5060245635 @default.
- W2183630672 hasAuthorship W2183630672A5060245983 @default.
- W2183630672 hasConcept C100012643 @default.
- W2183630672 hasConcept C10147152 @default.
- W2183630672 hasConcept C104317684 @default.
- W2183630672 hasConcept C140704245 @default.
- W2183630672 hasConcept C153911025 @default.
- W2183630672 hasConcept C159047783 @default.
- W2183630672 hasConcept C22744801 @default.
- W2183630672 hasConcept C2522874641 @default.
- W2183630672 hasConcept C2776650774 @default.
- W2183630672 hasConcept C33161422 @default.
- W2183630672 hasConcept C39060996 @default.
- W2183630672 hasConcept C40767141 @default.
- W2183630672 hasConcept C54355233 @default.
- W2183630672 hasConcept C81089528 @default.
- W2183630672 hasConcept C86803240 @default.
- W2183630672 hasConcept C92087593 @default.
- W2183630672 hasConceptScore W2183630672C100012643 @default.
- W2183630672 hasConceptScore W2183630672C10147152 @default.
- W2183630672 hasConceptScore W2183630672C104317684 @default.
- W2183630672 hasConceptScore W2183630672C140704245 @default.
- W2183630672 hasConceptScore W2183630672C153911025 @default.
- W2183630672 hasConceptScore W2183630672C159047783 @default.
- W2183630672 hasConceptScore W2183630672C22744801 @default.
- W2183630672 hasConceptScore W2183630672C2522874641 @default.
- W2183630672 hasConceptScore W2183630672C2776650774 @default.
- W2183630672 hasConceptScore W2183630672C33161422 @default.
- W2183630672 hasConceptScore W2183630672C39060996 @default.
- W2183630672 hasConceptScore W2183630672C40767141 @default.
- W2183630672 hasConceptScore W2183630672C54355233 @default.
- W2183630672 hasConceptScore W2183630672C81089528 @default.
- W2183630672 hasConceptScore W2183630672C86803240 @default.
- W2183630672 hasConceptScore W2183630672C92087593 @default.
- W2183630672 hasIssue "4" @default.
- W2183630672 hasLocation W21836306721 @default.
- W2183630672 hasOpenAccess W2183630672 @default.
- W2183630672 hasPrimaryLocation W21836306721 @default.
- W2183630672 hasRelatedWork W1487857142 @default.
- W2183630672 hasRelatedWork W1970057644 @default.
- W2183630672 hasRelatedWork W2030292786 @default.
- W2183630672 hasRelatedWork W2088538321 @default.
- W2183630672 hasRelatedWork W2183630672 @default.
- W2183630672 hasRelatedWork W2288514055 @default.
- W2183630672 hasRelatedWork W2374714249 @default.
- W2183630672 hasRelatedWork W2386002300 @default.
- W2183630672 hasRelatedWork W4232860627 @default.
- W2183630672 hasRelatedWork W4254148418 @default.
- W2183630672 hasVolume "31" @default.
- W2183630672 isParatext "false" @default.
- W2183630672 isRetracted "false" @default.
- W2183630672 magId "2183630672" @default.
- W2183630672 workType "article" @default.