Matches in SemOpenAlex for { <https://semopenalex.org/work/W2183637382> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2183637382 endingPage "199" @default.
- W2183637382 startingPage "194" @default.
- W2183637382 abstract "Background: Echinococcus granulosus is a cestode parasite that causes cystic hydatid disease in humans worldwide. The gene encoding EG95 protein may be a good candidate to design a DNA vaccine to prevent the disease. Considering the importance of EG95 gene and the scarceness of research on it in Iran, this study was carried out to determine and clone the gene encoding EG95 from Iranian isolate of E. granulosus. Materials and Methods: At the first stage, protoscoleces was isolated from hydatid cyst fluid and then RNA was extracted from protoscoleces and after performing RT-PCR, the amplified cDNA samples were detected by gel electrophoresis. In next stage, the obtained gene was cloned in pTZ57R/T vector. Two methods were used for conformation of cloning: colony PCR amplification and digestion with the EcoRI and XhoI restriction enzymes. Finally, the cloned EG95 gene in pTZ57R/T vector was sequenced.Results: Homological comparison of sequences showed that cDNA of EG95 in Iranian isolate of E. granulosus had 492 bp and was different from the standard strain of EG95 reported from New Zealand and Australia (X90928.1). Moreover, cloning of EG95 gene in pTZ57R/T plasmid was confirmed by digestion of this plasmid with the restriction enzymes. Conclusion: The EG95 gene was cloned in pTZ57R/T plasmid successfully and this plasmid can be used to design a DNA vaccine in further studies. Keywords: Echinococcus granulosus, EG95 gene, Protoscoleces, pTZ57R/T plasmid," @default.
- W2183637382 created "2016-06-24" @default.
- W2183637382 creator A5000311128 @default.
- W2183637382 creator A5006720918 @default.
- W2183637382 creator A5071216900 @default.
- W2183637382 creator A5072366432 @default.
- W2183637382 date "2011-09-15" @default.
- W2183637382 modified "2023-09-24" @default.
- W2183637382 title "Determination and cloning of the gene encoding EG95 protein in Iranian isolate of Echinococcus granulosus" @default.
- W2183637382 cites W1985504233 @default.
- W2183637382 cites W1991047145 @default.
- W2183637382 cites W2021623904 @default.
- W2183637382 cites W2056248153 @default.
- W2183637382 cites W2082730102 @default.
- W2183637382 cites W2083916601 @default.
- W2183637382 cites W2104841713 @default.
- W2183637382 cites W2167874335 @default.
- W2183637382 cites W2186348475 @default.
- W2183637382 cites W2601713671 @default.
- W2183637382 hasPublicationYear "2011" @default.
- W2183637382 type Work @default.
- W2183637382 sameAs 2183637382 @default.
- W2183637382 citedByCount "0" @default.
- W2183637382 crossrefType "journal-article" @default.
- W2183637382 hasAuthorship W2183637382A5000311128 @default.
- W2183637382 hasAuthorship W2183637382A5006720918 @default.
- W2183637382 hasAuthorship W2183637382A5071216900 @default.
- W2183637382 hasAuthorship W2183637382A5072366432 @default.
- W2183637382 hasConcept C104317684 @default.
- W2183637382 hasConcept C121050878 @default.
- W2183637382 hasConcept C128319531 @default.
- W2183637382 hasConcept C130123407 @default.
- W2183637382 hasConcept C153911025 @default.
- W2183637382 hasConcept C187882448 @default.
- W2183637382 hasConcept C199360897 @default.
- W2183637382 hasConcept C22744801 @default.
- W2183637382 hasConcept C2777028278 @default.
- W2183637382 hasConcept C2778972215 @default.
- W2183637382 hasConcept C40767141 @default.
- W2183637382 hasConcept C41008148 @default.
- W2183637382 hasConcept C54355233 @default.
- W2183637382 hasConcept C86803240 @default.
- W2183637382 hasConcept C90856448 @default.
- W2183637382 hasConcept C92087593 @default.
- W2183637382 hasConceptScore W2183637382C104317684 @default.
- W2183637382 hasConceptScore W2183637382C121050878 @default.
- W2183637382 hasConceptScore W2183637382C128319531 @default.
- W2183637382 hasConceptScore W2183637382C130123407 @default.
- W2183637382 hasConceptScore W2183637382C153911025 @default.
- W2183637382 hasConceptScore W2183637382C187882448 @default.
- W2183637382 hasConceptScore W2183637382C199360897 @default.
- W2183637382 hasConceptScore W2183637382C22744801 @default.
- W2183637382 hasConceptScore W2183637382C2777028278 @default.
- W2183637382 hasConceptScore W2183637382C2778972215 @default.
- W2183637382 hasConceptScore W2183637382C40767141 @default.
- W2183637382 hasConceptScore W2183637382C41008148 @default.
- W2183637382 hasConceptScore W2183637382C54355233 @default.
- W2183637382 hasConceptScore W2183637382C86803240 @default.
- W2183637382 hasConceptScore W2183637382C90856448 @default.
- W2183637382 hasConceptScore W2183637382C92087593 @default.
- W2183637382 hasIssue "3" @default.
- W2183637382 hasLocation W21836373821 @default.
- W2183637382 hasOpenAccess W2183637382 @default.
- W2183637382 hasPrimaryLocation W21836373821 @default.
- W2183637382 hasRelatedWork W2021623904 @default.
- W2183637382 hasRelatedWork W2057698050 @default.
- W2183637382 hasRelatedWork W2118641780 @default.
- W2183637382 hasRelatedWork W2129234877 @default.
- W2183637382 hasRelatedWork W2135906095 @default.
- W2183637382 hasRelatedWork W2339881787 @default.
- W2183637382 hasRelatedWork W2356185750 @default.
- W2183637382 hasRelatedWork W2356486119 @default.
- W2183637382 hasRelatedWork W2369280407 @default.
- W2183637382 hasRelatedWork W2369846639 @default.
- W2183637382 hasRelatedWork W2373026734 @default.
- W2183637382 hasRelatedWork W2376622204 @default.
- W2183637382 hasRelatedWork W2382014042 @default.
- W2183637382 hasRelatedWork W2387659721 @default.
- W2183637382 hasRelatedWork W2393233201 @default.
- W2183637382 hasRelatedWork W2398919424 @default.
- W2183637382 hasRelatedWork W2407079277 @default.
- W2183637382 hasRelatedWork W2761444518 @default.
- W2183637382 hasRelatedWork W3088348974 @default.
- W2183637382 hasRelatedWork W3145181047 @default.
- W2183637382 hasVolume "15" @default.
- W2183637382 isParatext "false" @default.
- W2183637382 isRetracted "false" @default.
- W2183637382 magId "2183637382" @default.
- W2183637382 workType "article" @default.