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- W2183645356 abstract "This study was conducted to clarify the effect of the synthetic glucocorticoid dexamethasone (DEX) on the developmental competence of buffalo oocytes in vitro, two experiments were conducted. In experiment 1, buffalo cumulus-oocyte-complexes (COCs) were exposed during in vitro maturation (IVM) to 0.0 (control) or 1.0, 10, 50, 100 µg/ml of DEX. COCs were IVM in TCM-199 + 10% FCS, 10 µg/ml FSH, 50 µg/ml gentamicin. IVM was performed at 38.5°C for 24 h under 5%CO in humidified air. After IVM, cumulus cells were removed 2 by gentile pipetting, then fixed in 1:3 (v/v) Acetic acid: ethanol then stained with 1% aceto-orcine for detection of the nuclear configuration. The percentage of oocytes reaching the metaphase II (M II) stage (nuclear maturation) was recorded. In experiment 2, IVM was performed as in experiment 1 then the matured oocytes were in vitro fertilized (IVF) in presence of the same concentrations of DEX, in vitro matured buffalo oocytes were in vitro fertilized (IVF). After 18 h of oocyte-sperm co-incubation, the presence of first and second polar bodies as evidence of fertilization was detected under microscope. Results indicated that the percentage of buffalo oocytes reaching M II stage was significantly higher (P<0.05) in control than DEX treated groups. DEX even at the lowest concentration (1.0 µg/ml) caused oocyte activation (presence of two polar bodies and spontaneous cleavage). Fertilization rate was significantly higher (P<0.05) in control compared with the DEX treated groups in a dose dependent manner. Conclusion, based on these in vitro findings, DEX has an influence on buffalo oocyte maturation and subsequent fertilization rate." @default.
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- W2183645356 date "2014-01-01" @default.
- W2183645356 modified "2023-09-30" @default.
- W2183645356 title "Effect of Dexamethasone on In vitro Maturation and Subsequent Fertilization of Buffalo Oocytes" @default.
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