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• W2183650215 abstract "HomePlant DiseaseVol. 100, No. 2First Report of Pythium spinosum Causing Root Rot of Chili (Capsicum annuum) in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pythium spinosum Causing Root Rot of Chili (Capsicum annuum) in PakistanK. Nawaz, A. A. Shahid, M. N. Subhani, W. Anwar, and M. AslamK. NawazSearch for more papers by this author, A. A. ShahidSearch for more papers by this author, M. N. SubhaniSearch for more papers by this author, W. AnwarSearch for more papers by this author, and M. AslamSearch for more papers by this authorAffiliationsAuthors and Affiliations K. Nawaz A. A. Shahid M. N. Subhani W. Anwar , Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan M. Aslam , Directorate General of Pest Warning and Quality Control of Pesticides, Punjab, Lahore. Published Online:1 Dec 2015https://doi.org/10.1094/PDIS-07-15-0745-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In September 2014, wilting of chili (Capsicum annuum L.) plants was observed during a survey of chili growing areas of the Kasur District (31.12° N; 74.45° E) in Punjab Province, Pakistan. Symptoms of root rot appeared at all stages of plant growth under warm and wet environmental conditions. Stems of affected plants developed a dark and shriveled portion at the soil line. Stem lesions were dark green, water-soaked, and roots turned dark brown. Entire fields were severely affected by the disease. Soil and root samples were collected for pathogen isolation. Root portions were cut into small pieces and surface sterilized by 1% sodium hypochlorite for 3 to 5 min, followed by rinsing in sterile distilled water. The tissue was placed onto corn meal agar (CMA) media supplemented with pimaricin (10 mg/liter), ampicillin 250 (mg/liter), and rifampicin (10 mg/liter) and incubated at 20°C in the dark. After one week of incubation, arachnoid-cottony white colonies appeared. Main hyphae were nonseptate, globose or limoniform, thin-walled, ranging in size from 2.7 to 4.8 μm in diameter with terminal and intercalary hyphal swellings. Hyphae occasionally had 2 to 3 digitate protuberances. Oogonia were produced terminally, globose, and 15 to 20 μm in diameter. Oospores were plerotic, rarely aplerotic, thin-walled, and 16 to 19 μm wide (Levesque and De Cock 2004). The pathogen was identified as Pythium spinosum based on its morphological characteristics and submitted to First Fungal Culture Bank of Pakistan (FCBP; Accession No. 1447). Total DNA was extracted using a CTAB method (Doyle and Doyle 1987) and the internal transcribed spacer region (ITS) was amplified with ITS1 and ITS4 primers (White et al. 1990). Amplified product was purified, sequenced, and submitted to NCBI (National Center for Biotechnology Information) database with GenBank (LN867319). This sequence of 942 bp showed 99% homology with Accession Nos. KJ162353 and HQ643791 of P. spinosum isolated from China and India, respectively. Koch’s postulates were fulfill by inoculating one-month-old chili seedlings (variety High Fly-1) with a concentration of 1 × 106 zoospore/ml formed in distilled water. Zoospore suspension of 100 ml was applied to the rhizosphere of chili plants as a soil drench. Control plants received distilled sterilized water in the same quantity. Ten replicates plants were kept in each treatment and the experiment was repeated twice. Plants were kept in a greenhouse at 30°C for disease development. After 15 days, all inoculated plants showed the symptoms of wilting and stem lesions similar to those observed in naturally infected plants. Control plants remained healthy and showed no symptoms. The pathogen was reisolated and confirmed morphologically. Root rot diseases caused by different Pythium spp. have already been reported on many plant species worldwide (Saleem et al. 1996). To our knowledge, this is the first report of P. spinosum causing root rot of chili in Pakistan. This confirmation of root rot of chili in Pakistan illustrates the need for planting chili varieties with resistance to this disease in areas where it has been identified.References:Doyle, J. J., and Doyle, J. L. 1987. Phytochem. Bull. 19:11. Google ScholarLéVesque, C. A., and De Cock, A. W. A. M. 2004. Mycol. Res. 108:1363. https://doi.org/10.1017/S0953756204001431 Crossref, ISI, Google ScholarSaleem, A., et al. 1996. Pak. J. Bot. 9:80. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Innis, M. A., et al., eds. Academic Press, San Diego. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 2 February 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Feb 2016Published: 1 Dec 2015Accepted: 1 Sep 2015 Pages: 526-526 Information© 2016 The American Phytopathological SocietyCited byMorphological and molecular characterization of Pythium species from Punjab, Pakistan7 March 2023 | Archives of Phytopathology and Plant Protection, Vol. 35Biosynthesis of Zinc Nanocomplex Employing for Plant Growth Promotion and Bio-Control of Pythium ultimum6 July 2022Pythium spinosum (root rot: ornamentals)CABI Compendium, Vol. CABI CompendiumActivation of Capsicum annuum L. Defense System against Fusarium Wilt through Plant Activators and their Impact on Horticultural Attributes30 December 2021 | International Journal of Phytopathology, Vol. 10, No. 3Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management13 April 2021 | Microorganisms, Vol. 9, No. 4Vegetable grafting in promoting sustainable vegetable production in developing countriesActa Horticulturae, No. 1302A loop-mediated isothermal amplification assay can rapidly diagnose soybean root-rot and damping-off diseases caused by Pythium spinosum23 August 2019 | Australasian Plant Pathology, Vol. 48, No. 6Streptomyces griseocarneus R132 controls phytopathogens and promotes growth of pepper (Capsicum annuum)Biological Control, Vol. 138" @default.
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• W2183650215 title "First Report of <i>Pythium spinosum</i> Causing Root Rot of Chili (<i>Capsicum annuum</i>) in Pakistan" @default.
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