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- W2183669418 abstract "One of the more controversial and debatable issues presently confronting clinical virologists and infectious disease specialists is the application of the cytomegalovirus antigenemia (CMV-Ag) assay (pp65 antigenemia assay) as a marker and predictor of CMV disease. For example, recent reports have shown the CMV-Ag assay to be more accurate than conventional DNA PCR or the more recent nucleic acid sequencebased amplification (NASBA) as a predictor of CMV in patients with AIDS (2). In a second comparison study, the CMV-Ag assay was reported to be less accurate than the Digene Capture CM DNA System (Digene), a nongenome amplification assay, as a predictor of CMV disease in a similar (AIDS) patient population (13). As recently pointed out by several researchers (13, 14), differences in the purported efficacy of the CMV-Ag assay might reflect such factors as antiviral management (prophylaxis against CMV versus no prophylaxis), duration of patient observation (e.g., 8 versus 12 months), the number of CD4 1 T-lymphocyte counts at patient entry into a given study, the monitoring of CMV disease other than retinitis, and/or the laboratory protocol used in the performance of the CMV-Ag assay per se. Specifically, and perhaps most technically important, data ascertained from CMV-Ag assay evaluation testing might be skewed in relation to the input polymorphonuclear leukocyte (PMNL) concentration utilized in the preparation of the cytospot. Wattanamano and colleagues (13) compared three assays (the CMV-Ag assay [Digene] and the AMPLICOR Qualitative PCR Test [Roche] as markers for the early detection of CMV infection in AIDS patients. Both the Digene and Roche assays were performed as described in the manufacturers’ specifications. However, the CMV-Ag assay was modified from that of" @default.
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- W2183669418 date "2000-01-01" @default.
- W2183669418 modified "2023-09-26" @default.
- W2183669418 title "Leukocyte Concentration in the Performance of the pp65 Antigenemia Assay" @default.
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