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• W2183678352 abstract "Background: Signal transducer and activator of transcription 3 (Stat3) is a member of the Janus-activated kinase(Jak)/ Stat signaling pathway. Abnormal activation of Stat3 plays a critical role in metastasis and invasion in varieties of human tumors including pancreatic cancer. This study aimed to investigate the mechanisms of activation and blocking of the Stat3 signaling pathway and its effects on invasion and metastasis of human pancreatic cancer cells. Methods: The Jak inhibitor AG490 and interleukin-6 (IL-6) were added to the culture media of human pancreatic cancer cells SW1990 and Capan-2, respectively. Cell growth was measured by MTT assays. Western blotting and immunocytochemistry were performed to detect phosphorylated Stat3 (p-Stat3) protein, while VEGF and MMP-2 mRNA and protein expression were examined with fluorescence quantitative polymerase chain reaction and Western blotting, respectively. The invasion ability of SW1990 and Capan-2 cells was determined by cell invasion assay." @default.
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• W2183678352 date "2010-01-01" @default.
• W2183678352 modified "2023-09-22" @default.
• W2183678352 title "signaling pathway and invasion of human pancreatic cancer cells in vitro" @default.
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