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- W2183687997 abstract "Stem cells have the ability to congregate at wound sites to repair damaged tissue. Design of 3D scaffolds that mimic the in vivo physical and chemical characteristics of human tissue and also facilitate proper survival, proliferation, and differentiation of stem cells proves challenging. Stem cell migration within such a scaffold is critical for tissue restoration. The goal is to gather enough data about cell movement in 2D to create an accurate model to describe and predict cell movement in 3D. To achieve this, polyacrylamide hydrogels are created that vary in stiffness and adhesivity. The gels are seeded with dyed cells and a microscope is set to take pictures of the cells every 15 minutes for 12 hours. Each cell can be tracked throughout the resulting movie with the aid of the computer program Imaris. The data about each cell’s movement can be run in MATLAB to find average cell speed, mean path length, total displacement, persistence, and chemotactic information. The data indicates that cells move farthest on softer gels under both protein conditions. Further research is required regarding how these cells move on topographical gels instead of flat gels to test how the radius of curvature (a 3D parameter) dictates cell movement." @default.
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- W2183687997 date "2011-01-01" @default.
- W2183687997 modified "2023-09-27" @default.
- W2183687997 title "Stem Cell Migration on 2D Biomaterials Platforms" @default.
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