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- W2183698015 abstract "The Scatchard plot analysis of the binding of 3H—PGEi to Leydig cells of rat testes revealed heterogeneity of the binding sites. Kinetic studies indicated that such a heterogeneity is not caused by negative cooperativity but is rather due to the presence of different binding sites characterized by Kai = 2.2x 108 mol~'l and Ka2 = 8.7x 107mol_11. The specific PGE, binding increased up to the 50th postnatal day and then decreased gradually. The changes in the binding capacity of Leydig cells did not correlate with the responsiveness to PGEi in cAMP and testosterone production. The highest responsiveness of Leydig cells to PGE) in cAMP synthesis was found during the first postnatal month, in testosterone synthesis at the age of 50 days. Seven-day treatment of rats with 30 IU PMSG, 100 IU hCG, 1 mg testosterone and 100 (ig estradiol caused a reduction in 3H—PGEi binding to Leydig cells. One mg of Cortisol applied for seven days had a stimulatory effect, whereas 6 IU of ACTH did not change the PGEi binding. Plasma levels of testosterone were increased after long-term administration of PMSG and hCG, remained unchanged in ACTH and Cortisol treated rats, and were markedly reduced in animals injected with estradiol." @default.
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- W2183698015 date "1982-01-01" @default.
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- W2183698015 title "Interaction of Prostaglandin E, with Specific Receptors in Rat" @default.
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