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- W2183737877 abstract "A simple, precise, specific and accurate Reverse phase HPLC method has been developed for the determination of Pyrazinamide in bulk and pharmaceutical dosage forms. Chromatography was performed on a Hypersil C8 (4.6 x 250mm, 3.5 μ m) column with Phosphate buffer (pH 4.4): Methanol 80:20 (v/v) as a mobile phase at a flow rate of 1 ml/ min. Detection was performed at 269 nm. The retention time of Pyrazinamide was found to be 3.62min. By adoption of this procedure Pyrazinamide (PYZ) is eluted completely. Linear calibration plots were obtained between 20- 120µg/ml.The method of analysis was used for quantification of PYZ in pharmaceutical preparations with a coefficient of variation <2%. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, ruggedness and robustness." @default.
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- W2183737877 date "2011-01-01" @default.
- W2183737877 modified "2023-09-27" @default.
- W2183737877 title "Development and Validation of RP-HPLC Method for Quantitative estimation of Pyrazinamide in Bulk and Pharmaceutical dosage forms" @default.
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