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- W2183782085 abstract "Objective: Present study deals with the isolation of bioactive component juglone from stem bark extracts of J. regia and its quantification by a widely used HPTLC method. Methods: Juglans regia stem bark was powdered and extracted using non polar to polar solvents. The potent molecule juglone was achieved by executing chromatographic technique. Its structure was confirmed by analysis of spectral data. A precise analytical technique (HPTLC) for the quantification was manifested. Results: Stem bark extracts with solvents chloroform(A), ethyl acetate(B), acetone(C), ethanol(D) and methanol(E) were analyzed chromatographically to achieve 5-hydroxy-1,4-naphthaquinone (Juglone) as yellow crystalline needles. Quantification of this compound from extracts (A-E) was performed. The results of HPTLC experiment reveal that the acetone extract is rich (151.0µg/gm) in this bioactive compound whereas methanol extract contains least amount (29.34µg/gm) of it. Conclusion: 5- Hydroxy 1, 4- Naphthaquinone (Juglone) had been isolated from the acetone extract of J. regia stem bark. This biologically active compound was estimated quantitatively from various bark extracts by HPTLC method. The proposed method was found to be simple, rapid, accurate, precise, economic, reliable and reproducible." @default.
- W2183782085 created "2016-06-24" @default.
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- W2183782085 date "2013-01-01" @default.
- W2183782085 modified "2023-09-27" @default.
- W2183782085 title "QUANTIFICATION OF A PHYTOTOXIN FROM WALNUT SPECIES BY HPTLC METHOD" @default.
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