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- W2183909725 abstract "Spectroscopy at the single-molecule level is a very useful technique for revealing molecular dynamics that are averaged out in bulk measurements. Absorption spectroscopy has several advantages over the more common fluorescence-based single-molecule studies: it eliminates shelving of fluorescenct molecules and does not require fluorescenct tags, making it a far less instrusive method. However, absorption measurements suffer from a high noise-background and rather low sensitivity. In our approach to absorption spectroscopy, a Fabry-Perot optical cavity greatly enhances the interaction between the photons and the molecules under study (in this case, bacteriochlorophyll-a, BChla ), resulting in higher sensitivity. This paper describes the design of the cavity and the optical excitation and measurement apparatus, as well as first results of absorption measurements. The high-finesse cavity consists of closely spaced (∼ 100μm) mirrors, giving it wide free spectral range and fast response time. The cavity is scannable by piezoelectric actuators for mode-locking. It is contained in a chemically stable chamber that can be filled with the desired solution—in the case of these early measurements, with BChla in acetone. For easy operation, the cavity is flushable with a pumping apparatus. The cavity mode volume is minimized for measurments on small numbers of molecules. In this project, preliminary absorption measurements were performed on as little as 106 BChla molecules. It is estimated that this cavity can be used to make absorption measurements of less than ten molecules if other noise factors are reduced. The longer-term goal is to observe conformational changes in BChla while it is embedded in the cell membrane. In the more distant future, this method could be used to observe, in real time, many types of organic molecules as they undergo conformational changes in vitro. Combined with knowledge obtained through crystallography and direct mutagenesis, this information would greatly improve our understanding of many kinds of organic complexes and the processes in which they take part." @default.
- W2183909725 created "2016-06-24" @default.
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- W2183909725 date "2002-01-01" @default.
- W2183909725 modified "2023-09-24" @default.
- W2183909725 title "Cavity-Enhanced Absorption Spectroscopy of BChla" @default.
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