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- W2184164805 abstract "Fundamental knowledge of sperm cryosensitivity is required to establish sperm cryopreservation techniques for the genetic management of a threatened canid, the maned wolf (Chrysocyon brachyurus). The objective of this study was to determine the osmotic tolerance of maned wolf spermatozoa. Semen was electroejaculated from 12 male maned wolves (n = 13 ejaculates) during the North American breeding season (October February) and analyzed for motility, forward progressive status, morphology, viability, osmolality, pH, volume, and total sperm. Suitable samples (n = 7; motility > 40%, total sperm > 0.3 x 10 6 ) were washed, re-suspended in Ham’s F10 medium supplemented with bovine serum albumin (BSA) and exposed to hypertonic solutions of NaCl (350, 400, 600, 800 and 1000 mOsm) and cryoprotectants (CPA’s; 0.1 M sucrose, 0.2 M sucrose, 1 M glycerol and 1 M dimethyl sulfoxide (DMSO) in addition to a control (300mOsm) for 10 min. After dilution back into isotonic medium (300 mOsm, 37 C), samples were assessed for motility and viability. Compared to the raw ejaculates, motility was affected (P 350 mOsm and by both solutions of sucrose. However, motility of sperm exposed to glycerol or DMSO was comparable (P > 0.05) to that of the raw ejaculate. Exposure of maned wolf spermatozoa to solutions of NaCl > 600 mOsm" @default.
- W2184164805 created "2016-06-24" @default.
- W2184164805 creator A5062272322 @default.
- W2184164805 date "2012-01-01" @default.
- W2184164805 modified "2023-09-24" @default.
- W2184164805 title "The Development of Assisted Reproductive Techniques for Managing Maned Wolves (Chrysocyon brachyurus) Ex situ A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at George Mason University" @default.
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