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- W2184199396 abstract "The effect and mechanism of IL-8 on A549 cells migration were investigated. The appropri-ate concentration of IL-8 was chosen by MTT assay. The Western-blot assay, Scratch test and Transwell assay were used in this study to analyse the effect and mechanism of IL-8 on lung adenocarcinoma A549 cells migration. The results of Western-blot assay showed that: (1) IL-8 can promote the expression of MMP-2 protein and has no effect on the expression of MMP-9. (2) IL-8 can promote the expression of JNK/SAPK phosphorylated protein. (3) In-hibitor (SP600125) can block the effect of IL-8 on the expression of MMP-2 protein. Scratch test proved from the opposite side that the low expression of MMP-2 could inhibit the mi-gration of A549 cells. These results suggest that IL-8 can regulate MMP-2 protein expression through the JNK/SAPK signaling pathway and further promote the migration of lung ade-nocarcinoma A549 cells." @default.
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- W2184199396 date "2014-01-01" @default.
- W2184199396 modified "2023-09-26" @default.
- W2184199396 title "An experimental Study on the mechanism for IL-8 promoting migration of A549 cells." @default.
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