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- W2184310391 abstract "The study highlights that, SYBR Green real-time PCR test is a rapid, reproducible and robust method for detecting Salmonella enteritidis, Salmonella Typhimurium and Salmonella Kentucky in poultry samples using primers SEFA-2 and SEFA-4 for S. enteritidis, fli 15 and tym for S. Typhimurium and S. kentucky, respectively. The specificity of the reaction was confirmed by the melting temperature (Tm), which was consistently specific for the amplicon obtained; the mean peak Tm obtained with curves specific for serotype Enteritidis was 83.03°C while it was 83.99°C and 84.01°C for S. Kentucky and S. Typhimurium, respectively. The results of this study demonstrate that the SYBR Green I real-time PCR constitutes an effective and easy-to-perform method for detecting S. enteritidis, S. Typhimurium and S. kentucky in poultry samples." @default.
- W2184310391 created "2016-06-24" @default.
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- W2184310391 date "2014-01-01" @default.
- W2184310391 modified "2023-09-23" @default.
- W2184310391 title "Application of SYBR Green Real-Time PCR to Detect Salmonella enterica Serotype Enteritidis, Typhimurium and Kentucky in Poultry" @default.
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