Matches in SemOpenAlex for { <https://semopenalex.org/work/W2184588217> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2184588217 endingPage "63" @default.
- W2184588217 startingPage "57" @default.
- W2184588217 abstract "Homogenates of bovine adrenal medullae hydrolyzed exogenous sphingomyelin at 4.3 +/- 1.6 nmol X mg-1 X min-1 and 97% of this sphingomyelinase activity was sedimentable at 110,000 g. The sphingomyelinase had a broad pH optimum centered at pH 7. Enzymatic activity was maximal with 80 microM added Mn2+; Mg2+ supported less than half maximal activity and both Ca2+ and EDTA inhibited activity. No activity was detected in the absence of Triton X-100. Response to detergent was biphasic with dose-dependent stimulation from 0.02% to 0.05% Triton X-100 followed by inhibition with increasing concentrations of detergent. Activity in response to detergent was also modulated by protein concentration. Sphingomyelinase activity was associated with a plasma membrane-microsomal fraction. Phosphatidylcholine was not hydrolyzed under optimal conditions for sphingomyelin hydrolysis and a variety of other conditions. Neutral-active sphingomyelinase activity in adrenal medulla was similar in magnitude to that observed in other non-neural bovine tissues. This study demonstrates the presence of a potent neutral-active sphingomyelinase in a plasma membrane-microsomal fraction of bovine adrenal medulla. This enzyme may be involved in membrane fusion and lysis during catecholamine secretion through its ability to alter membrane composition." @default.
- W2184588217 created "2016-06-24" @default.
- W2184588217 creator A5003252664 @default.
- W2184588217 creator A5086841343 @default.
- W2184588217 date "1986-11-01" @default.
- W2184588217 modified "2023-09-27" @default.
- W2184588217 title "Characterization and localization of neutral sphingomyelinase in bovine adrenal medulla." @default.
- W2184588217 cites W1486290010 @default.
- W2184588217 cites W1496005353 @default.
- W2184588217 cites W1526385127 @default.
- W2184588217 cites W1530654169 @default.
- W2184588217 cites W1553510508 @default.
- W2184588217 cites W1595192741 @default.
- W2184588217 cites W1599947302 @default.
- W2184588217 cites W190883241 @default.
- W2184588217 cites W1969805452 @default.
- W2184588217 cites W1971048984 @default.
- W2184588217 cites W1973417166 @default.
- W2184588217 cites W1974860806 @default.
- W2184588217 cites W1981841638 @default.
- W2184588217 cites W1982857351 @default.
- W2184588217 cites W1985342306 @default.
- W2184588217 cites W1992377220 @default.
- W2184588217 cites W2002873221 @default.
- W2184588217 cites W2027127398 @default.
- W2184588217 cites W2038162319 @default.
- W2184588217 cites W2051254820 @default.
- W2184588217 cites W2069370714 @default.
- W2184588217 cites W2093835809 @default.
- W2184588217 cites W2095247814 @default.
- W2184588217 cites W2106463709 @default.
- W2184588217 cites W2113979788 @default.
- W2184588217 cites W2142539904 @default.
- W2184588217 cites W2157978273 @default.
- W2184588217 cites W4293247451 @default.
- W2184588217 doi "https://doi.org/10.1016/s0022-2275(20)38854-4" @default.
- W2184588217 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3007647" @default.
- W2184588217 hasPublicationYear "1986" @default.
- W2184588217 type Work @default.
- W2184588217 sameAs 2184588217 @default.
- W2184588217 citedByCount "18" @default.
- W2184588217 countsByYear W21845882172023 @default.
- W2184588217 crossrefType "journal-article" @default.
- W2184588217 hasAuthorship W2184588217A5003252664 @default.
- W2184588217 hasAuthorship W2184588217A5086841343 @default.
- W2184588217 hasBestOaLocation W21845882171 @default.
- W2184588217 hasConcept C126322002 @default.
- W2184588217 hasConcept C134018914 @default.
- W2184588217 hasConcept C17291353 @default.
- W2184588217 hasConcept C185592680 @default.
- W2184588217 hasConcept C2775894120 @default.
- W2184588217 hasConcept C2777597313 @default.
- W2184588217 hasConcept C2778163477 @default.
- W2184588217 hasConcept C55493867 @default.
- W2184588217 hasConcept C61322309 @default.
- W2184588217 hasConcept C71924100 @default.
- W2184588217 hasConcept C86803240 @default.
- W2184588217 hasConceptScore W2184588217C126322002 @default.
- W2184588217 hasConceptScore W2184588217C134018914 @default.
- W2184588217 hasConceptScore W2184588217C17291353 @default.
- W2184588217 hasConceptScore W2184588217C185592680 @default.
- W2184588217 hasConceptScore W2184588217C2775894120 @default.
- W2184588217 hasConceptScore W2184588217C2777597313 @default.
- W2184588217 hasConceptScore W2184588217C2778163477 @default.
- W2184588217 hasConceptScore W2184588217C55493867 @default.
- W2184588217 hasConceptScore W2184588217C61322309 @default.
- W2184588217 hasConceptScore W2184588217C71924100 @default.
- W2184588217 hasConceptScore W2184588217C86803240 @default.
- W2184588217 hasIssue "1" @default.
- W2184588217 hasLocation W21845882171 @default.
- W2184588217 hasOpenAccess W2184588217 @default.
- W2184588217 hasPrimaryLocation W21845882171 @default.
- W2184588217 hasRelatedWork W1986362058 @default.
- W2184588217 hasRelatedWork W1987379489 @default.
- W2184588217 hasRelatedWork W1998227988 @default.
- W2184588217 hasRelatedWork W2004734051 @default.
- W2184588217 hasRelatedWork W2006062886 @default.
- W2184588217 hasRelatedWork W2014896485 @default.
- W2184588217 hasRelatedWork W2028632688 @default.
- W2184588217 hasRelatedWork W2068263482 @default.
- W2184588217 hasRelatedWork W2072908520 @default.
- W2184588217 hasRelatedWork W2080670130 @default.
- W2184588217 hasVolume "27" @default.
- W2184588217 isParatext "false" @default.
- W2184588217 isRetracted "false" @default.
- W2184588217 magId "2184588217" @default.
- W2184588217 workType "article" @default.