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• W2184694510 abstract "Objectives: To evaluate, in vitro, the effectiveness of different concentrations of sodium hypochlorite [NaOCl] (0.5 %, 1.0 %, 2.5% and 5.25% w/v) in the elimination of Enterococcus faecalis E10e. Methods: E. faecalis E10e was grown overnight and allowed to grow until the ‘mid-exponential’ phase. 0.2 ml of the inoculum that was then transferred into 9.8 ml of NaOCl solution. One ml samples were removed and poured in 9 ml of sodium thiosulphate [Na2S2O3] solution (neutralisation) after 30 sec, 1 min, 2 min, 5 min, 10 min and 30 min. Serial dilution of each neutralised solution was carried out to give a dilution factor of 10 -6 . Subsequently, 0.02 ml of each dilution was plated onto an agar plate, and then incubated at 37C in an aerobic incubator for 24 hrs. The number of viable colonies (Colony Forming Units / ml) was determined for each plate. Results: The results showed that 5.25 % NaOCl was the most effective irrigant assessed, killing 100% of bacterial cells in 2 min. However, the time required by 2.5 %, 1.0 % and 0.5% concentrations was 5 min, 10 min and 30 min, respectively. Conclusion: There was a statistically significant difference between NaOCl concentrations with respect to the mean number of viable counts recovered, with 5.25 % NaOCl being the most effective irrigant assessed. There was a strong curvilinear relationship between NaOCl concentrations and time taken to attain zero viable counts (100 % killing)." @default.
• W2184694510 created "2016-06-24" @default.
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• W2184694510 date "2012-01-01" @default.
• W2184694510 modified "2023-09-27" @default.
• W2184694510 title "An In Vitro Evaluation of the Influence of Concentration and Duration of Exposure on the Antimicrobial Efficacy of Sodium Hypochlorite on Enterococcus Faecalis" @default.
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