FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2184817372> ?p ?o ?g. }

• W2184817372 abstract "Most β-glucosidases reported are sensitive to the end product (glucose), making it the rate limiting component of cellulase for efficient degradation of cellulose through enzymatic route. Thus, there are ongoing interests in searching for glucose-tolerant β-glucosidases, which are still active at high glucose concentration. Although many β-glucosidases with different glucose-tolerance levels have been isolated and characterized in the past decades, the effects of glucose-tolerance on the hydrolysis of cellulose are not thoroughly studied.In the present study, a novel β-glucosidase (Bgl6) with the half maximal inhibitory concentration (IC 50) of 3.5 M glucose was isolated from a metagenomic library and characterized. However, its poor thermostability at 50 °C hindered the employment in cellulose hydrolysis. To improve its thermostability, random mutagenesis was performed. A thermostable mutant, M3, with three amino acid substitutions was obtained. The half-life of M3 at 50 °C is 48 h, while that of Bgl6 is 1 h. The K cat/K m value of M3 is 3-fold higher than that of Bgl6. The mutations maintained its high glucose-tolerance with IC 50 of 3.0 M for M3. In a 10-h hydrolysis of cellobiose, M3 completely converted cellobiose to glucose, while Bgl6 reached a conversion of 80 %. Then their synergistic effects with the commercial cellulase (Celluclast 1.5 L) on hydrolyzing pretreated sugarcane bagasse (SCB) were investigated. The supplementation of Bgl6 or mutant M3 to Celluclast 1.5 L significantly improved the SCB conversion from 64 % (Celluclast 1.5 L alone) to 79 % (Bgl6) and 94 % (M3), respectively. To further evaluate the application potential of M3 in high-solids cellulose hydrolysis, such reactions were performed at initial glucose concentration of 20-500 mM. Results showed that the supplementation of mutant M3 enhanced the glucose production from SCB under all the conditions tested, improving the SCB conversion by 14-35 %.These results not only clearly revealed the significant role of glucose-tolerance in cellulose hydrolysis, but also showed that mutant M3 may be a potent candidate for high-solids cellulose refining." @default.
• W2184817372 created "2016-06-24" @default.
• W2184817372 creator A5001830020 @default.
• W2184817372 creator A5016375658 @default.
• W2184817372 creator A5027331800 @default.
• W2184817372 creator A5056055523 @default.
• W2184817372 creator A5061544635 @default.
• W2184817372 creator A5064564309 @default.
• W2184817372 creator A5076662455 @default.
• W2184817372 date "2015-12-01" @default.
• W2184817372 modified "2023-10-16" @default.
• W2184817372 title "Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration" @default.
• W2184817372 cites W1493541064 @default.
• W2184817372 cites W1506261873 @default.
• W2184817372 cites W1555518803 @default.
• W2184817372 cites W1620763267 @default.
• W2184817372 cites W1954202239 @default.
• W2184817372 cites W1966991293 @default.
• W2184817372 cites W1967680131 @default.
• W2184817372 cites W1969630046 @default.
• W2184817372 cites W1970325009 @default.
• W2184817372 cites W1984180361 @default.
• W2184817372 cites W1988837050 @default.
• W2184817372 cites W1994915593 @default.
• W2184817372 cites W1995836224 @default.
• W2184817372 cites W2005291574 @default.
• W2184817372 cites W2008185431 @default.
• W2184817372 cites W2012832696 @default.
• W2184817372 cites W2014958041 @default.
• W2184817372 cites W2018451578 @default.
• W2184817372 cites W2022899077 @default.
• W2184817372 cites W2027612040 @default.
• W2184817372 cites W2029314103 @default.
• W2184817372 cites W2043174582 @default.
• W2184817372 cites W2043660257 @default.
• W2184817372 cites W2063029585 @default.
• W2184817372 cites W2067344604 @default.
• W2184817372 cites W2068866569 @default.
• W2184817372 cites W2074916461 @default.
• W2184817372 cites W2084172626 @default.
• W2184817372 cites W2084973087 @default.
• W2184817372 cites W2089216334 @default.
• W2184817372 cites W2089643533 @default.
• W2184817372 cites W2092130903 @default.
• W2184817372 cites W2093728209 @default.
• W2184817372 cites W2105015569 @default.
• W2184817372 cites W2107769173 @default.
• W2184817372 cites W2109093244 @default.
• W2184817372 cites W2111870842 @default.
• W2184817372 cites W2116598854 @default.
• W2184817372 cites W2118855328 @default.
• W2184817372 cites W2124231317 @default.
• W2184817372 cites W2128836283 @default.
• W2184817372 cites W2131047404 @default.
• W2184817372 cites W2134145102 @default.
• W2184817372 cites W2134695519 @default.
• W2184817372 cites W2136038574 @default.
• W2184817372 cites W2149525061 @default.
• W2184817372 cites W2152108974 @default.
• W2184817372 cites W2156098543 @default.
• W2184817372 cites W2166137139 @default.
• W2184817372 cites W2171509237 @default.
• W2184817372 cites W2318958768 @default.
• W2184817372 cites W2326452486 @default.
• W2184817372 doi "https://doi.org/10.1186/s13068-015-0383-z" @default.
• W2184817372 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4666061" @default.
• W2184817372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26628916" @default.
• W2184817372 hasPublicationYear "2015" @default.
• W2184817372 type Work @default.
• W2184817372 sameAs 2184817372 @default.
• W2184817372 citedByCount "81" @default.
• W2184817372 countsByYear W21848173722016 @default.
• W2184817372 countsByYear W21848173722017 @default.
• W2184817372 countsByYear W21848173722018 @default.
• W2184817372 countsByYear W21848173722019 @default.
• W2184817372 countsByYear W21848173722020 @default.
• W2184817372 countsByYear W21848173722021 @default.
• W2184817372 countsByYear W21848173722022 @default.
• W2184817372 countsByYear W21848173722023 @default.
• W2184817372 crossrefType "journal-article" @default.
• W2184817372 hasAuthorship W2184817372A5001830020 @default.
• W2184817372 hasAuthorship W2184817372A5016375658 @default.
• W2184817372 hasAuthorship W2184817372A5027331800 @default.
• W2184817372 hasAuthorship W2184817372A5056055523 @default.
• W2184817372 hasAuthorship W2184817372A5061544635 @default.
• W2184817372 hasAuthorship W2184817372A5064564309 @default.
• W2184817372 hasAuthorship W2184817372A5076662455 @default.
• W2184817372 hasBestOaLocation W21848173721 @default.
• W2184817372 hasConcept C100544194 @default.
• W2184817372 hasConcept C134643618 @default.
• W2184817372 hasConcept C141603559 @default.
• W2184817372 hasConcept C150903083 @default.
• W2184817372 hasConcept C181199279 @default.
• W2184817372 hasConcept C185592680 @default.
• W2184817372 hasConcept C2776584326 @default.
• W2184817372 hasConcept C2779251873 @default.
• W2184817372 hasConcept C2780074830 @default.
• W2184817372 hasConcept C2780261296 @default.
• W2184817372 hasConcept C2780412358 @default.
• W2184817372 hasConcept C2780777981 @default.

• next