Matches in SemOpenAlex for { <https://semopenalex.org/work/W2184900292> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2184900292 abstract "Background : Dynamic changes in protein length (protein stretching events) have been observed in living cells using supra-resolution fluorescence microscopy. Our previous work documented the dynamic stretching of single molecules of talin, tagged with green fluorescent protein at the N-terminus and red fluorescent protein at the C- terminus (Margadant, et al., 2011). Previous speckle microscopy image analysis methods employed heuristics and assumptions that have not yet been analyzed for reliability. Methods : A CUDA-accelerated simulator was implemented to emulate the 2D image acquisition process of talin labeled with red and green fluorescent proteins. The molecular lengths obeyed a user-defined probability density distribution. Each fluorescence tag was given an emission probability based on published literature. Localization error was added to emulate the diffraction limit of the instrument. Collisions, or signals of the same color located within a collision radius, were detected with two-pass counting. Results : Four categories of assignments were studied in response to the following variable parameters: molecular density, collision radius, non-emitting tags, resolution limit, and molecular lengths. Jittering amplitude was found to have a profound impact on the observed histogram of molecular lengths. Conclusions : Simulation of image acquisition was able to define the limit of observability, and to characterize the breakdown of molecular length interpretation subject to high density, low resolution, or low emission rate." @default.
- W2184900292 created "2016-06-24" @default.
- W2184900292 creator A5031384457 @default.
- W2184900292 creator A5065180816 @default.
- W2184900292 creator A5080288956 @default.
- W2184900292 creator A5086214246 @default.
- W2184900292 date "2012-01-01" @default.
- W2184900292 modified "2023-09-27" @default.
- W2184900292 title "Characterization of Image Acquisition Noise in a Novel Fluorescence Speckle Microscopy Method" @default.
- W2184900292 hasPublicationYear "2012" @default.
- W2184900292 type Work @default.
- W2184900292 sameAs 2184900292 @default.
- W2184900292 citedByCount "0" @default.
- W2184900292 crossrefType "journal-article" @default.
- W2184900292 hasAuthorship W2184900292A5031384457 @default.
- W2184900292 hasAuthorship W2184900292A5065180816 @default.
- W2184900292 hasAuthorship W2184900292A5080288956 @default.
- W2184900292 hasAuthorship W2184900292A5086214246 @default.
- W2184900292 hasConcept C120665830 @default.
- W2184900292 hasConcept C121332964 @default.
- W2184900292 hasConcept C138268822 @default.
- W2184900292 hasConcept C147080431 @default.
- W2184900292 hasConcept C154945302 @default.
- W2184900292 hasConcept C169274487 @default.
- W2184900292 hasConcept C178635117 @default.
- W2184900292 hasConcept C185592680 @default.
- W2184900292 hasConcept C186060115 @default.
- W2184900292 hasConcept C38652104 @default.
- W2184900292 hasConcept C41008148 @default.
- W2184900292 hasConcept C86803240 @default.
- W2184900292 hasConcept C91881484 @default.
- W2184900292 hasConceptScore W2184900292C120665830 @default.
- W2184900292 hasConceptScore W2184900292C121332964 @default.
- W2184900292 hasConceptScore W2184900292C138268822 @default.
- W2184900292 hasConceptScore W2184900292C147080431 @default.
- W2184900292 hasConceptScore W2184900292C154945302 @default.
- W2184900292 hasConceptScore W2184900292C169274487 @default.
- W2184900292 hasConceptScore W2184900292C178635117 @default.
- W2184900292 hasConceptScore W2184900292C185592680 @default.
- W2184900292 hasConceptScore W2184900292C186060115 @default.
- W2184900292 hasConceptScore W2184900292C38652104 @default.
- W2184900292 hasConceptScore W2184900292C41008148 @default.
- W2184900292 hasConceptScore W2184900292C86803240 @default.
- W2184900292 hasConceptScore W2184900292C91881484 @default.
- W2184900292 hasLocation W21849002921 @default.
- W2184900292 hasOpenAccess W2184900292 @default.
- W2184900292 hasPrimaryLocation W21849002921 @default.
- W2184900292 hasRelatedWork W2013657823 @default.
- W2184900292 hasRelatedWork W2029049355 @default.
- W2184900292 hasRelatedWork W2032044504 @default.
- W2184900292 hasRelatedWork W2035215323 @default.
- W2184900292 hasRelatedWork W2043848880 @default.
- W2184900292 hasRelatedWork W2054363349 @default.
- W2184900292 hasRelatedWork W2054791523 @default.
- W2184900292 hasRelatedWork W2060921028 @default.
- W2184900292 hasRelatedWork W2066858190 @default.
- W2184900292 hasRelatedWork W2078079353 @default.
- W2184900292 hasRelatedWork W2104928963 @default.
- W2184900292 hasRelatedWork W2132982804 @default.
- W2184900292 hasRelatedWork W2241529370 @default.
- W2184900292 hasRelatedWork W2350377567 @default.
- W2184900292 hasRelatedWork W2469355012 @default.
- W2184900292 hasRelatedWork W2553441646 @default.
- W2184900292 hasRelatedWork W2734888091 @default.
- W2184900292 hasRelatedWork W2765660702 @default.
- W2184900292 hasRelatedWork W3006956294 @default.
- W2184900292 hasRelatedWork W653677542 @default.
- W2184900292 isParatext "false" @default.
- W2184900292 isRetracted "false" @default.
- W2184900292 magId "2184900292" @default.
- W2184900292 workType "article" @default.