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• W2184964717 abstract "The sequences encoding the mouse heavy-chain (VH) and light-chain (VL) variable region genes were isolated by PCR and joined into a single-chain Fv (scFv) DNA by using a DNA linker encoding (Gly4Ser)3 peptide. The scFv DNA fragment was cloned into the phagemid pCANTAB5E and expressed in Escherichia coli as a fusion protein with M13 phage p3 polypeptide and E tag. The scFv fusion protein was displayed on the surfaces of recombinant M13 phages in the presence of the helper phage M13K07. High-affinity scFv phage-bodies against citrus tristeza virus (CTV) were enriched through affinity selection on immobilized recombinant CTV coat protein preparations. The selected recombinant phages were used to infect Escherichia coli HB2151 for the production of soluble scFv antibodies. One selected clone in HB2151 secreted a soluble scFv antibody that detected CTV in extracts of infected citrus plants with a sensitivity comparable to that of a commercial monoclonal antibody. The nucleotide sequence of the light-chain and heavy-chain portions were closely related to other published scFv against CTV. The potential of this scFv was demonstrated in routine field testing using an inexpensive tissue print–ELISA." @default.
• W2184964717 created "2016-06-24" @default.
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• W2184964717 date "2007-01-01" @default.
• W2184964717 modified "2023-09-26" @default.
• W2184964717 title "Molecular cloning, expression, and characterization of mouse single-chain fragment variable antibody against Citrus tristeza virus" @default.
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