Matches in SemOpenAlex for { <https://semopenalex.org/work/W2184964767> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2184964767 abstract "Sakyo-ku, Kyoto606-8502 Japan Abstract: Inthis study, wedeveloped asingle molecule meas- Biomolecules, especially proteins, often consist ofseveral rigidurement technique withhightimeresolution byenhancing subunits andworklikemechanical devices. Therefore theirboththemeasurement andtheanalysis methods. We applied mechanical motion ortheconformational dynamics aresignifi- thedeveloped system toinvestigation oftheconformational cantly investigated withagreatdealofinterests. However, itis . .l.f.o achallenge todetect smallmechanical motion, especially when dynmcs infole folding e u ochromeec its dimension isassmall asseveral nanometers. Single molecule(cytc)protein molecules. Protein folding process hasbeen measurement technique makinguseoffluorescence resonance extensively investigated andistodateknowntoproceed by energy transfer (FRET)isoneofeffective solutions toobservewayofatleast afewintermediate states eveninthecaseof molecular dynamics inverysmall dimensions like nanometers. quitesmallproteins likecytc(2-3). Theensemble meas- We developed asingle molecule measurement technique withurements haveunveiled that cytchasatleast twointermedi- improved timeresolution. Weemployed thetimestampdetec-atestates. Thedetailed investigation bysingle molecule tion method toacquire thesignals including richinformation measurements iswaited. andthestatistical dataanalysis toextract essential information fromthesignal. Using thenewsystem, theconformational dy- 2.MATERIALS& METHODS namicsofcytochrome c(cyt c)protein molecules wasinvesti- gated.Thechangeofmolecular structure isdetected asthatof thefluorescence intensity bywayofFRETphenomenon. Some Inorder toachieve thehighly time-resolved measure- intermediate states wereresolved andthetimeresolution inthementsofsingle molecular conformational dynamics, we en- order ofmillisecond wasachieved. hanced boththeoptical measurement andthedataanalysis based onthecommonphoton counting-type single molecule" @default.
- W2184964767 created "2016-06-24" @default.
- W2184964767 creator A5012356285 @default.
- W2184964767 creator A5085607917 @default.
- W2184964767 date "2007-01-01" @default.
- W2184964767 modified "2023-09-27" @default.
- W2184964767 title "Realtime Measurement ofMechanical MotionofSingle Protein Molecules" @default.
- W2184964767 cites W1511345708 @default.
- W2184964767 hasPublicationYear "2007" @default.
- W2184964767 type Work @default.
- W2184964767 sameAs 2184964767 @default.
- W2184964767 citedByCount "0" @default.
- W2184964767 crossrefType "journal-article" @default.
- W2184964767 hasAuthorship W2184964767A5012356285 @default.
- W2184964767 hasAuthorship W2184964767A5085607917 @default.
- W2184964767 hasConcept C119599485 @default.
- W2184964767 hasConcept C121332964 @default.
- W2184964767 hasConcept C12554922 @default.
- W2184964767 hasConcept C127413603 @default.
- W2184964767 hasConcept C147597530 @default.
- W2184964767 hasConcept C159467904 @default.
- W2184964767 hasConcept C171250308 @default.
- W2184964767 hasConcept C178790620 @default.
- W2184964767 hasConcept C185592680 @default.
- W2184964767 hasConcept C192562407 @default.
- W2184964767 hasConcept C204328495 @default.
- W2184964767 hasConcept C2775981520 @default.
- W2184964767 hasConcept C2776545253 @default.
- W2184964767 hasConcept C32909587 @default.
- W2184964767 hasConcept C49853544 @default.
- W2184964767 hasConcept C55493867 @default.
- W2184964767 hasConcept C59593255 @default.
- W2184964767 hasConcept C62520636 @default.
- W2184964767 hasConcept C8010536 @default.
- W2184964767 hasConcept C86803240 @default.
- W2184964767 hasConcept C91881484 @default.
- W2184964767 hasConcept C96305047 @default.
- W2184964767 hasConceptScore W2184964767C119599485 @default.
- W2184964767 hasConceptScore W2184964767C121332964 @default.
- W2184964767 hasConceptScore W2184964767C12554922 @default.
- W2184964767 hasConceptScore W2184964767C127413603 @default.
- W2184964767 hasConceptScore W2184964767C147597530 @default.
- W2184964767 hasConceptScore W2184964767C159467904 @default.
- W2184964767 hasConceptScore W2184964767C171250308 @default.
- W2184964767 hasConceptScore W2184964767C178790620 @default.
- W2184964767 hasConceptScore W2184964767C185592680 @default.
- W2184964767 hasConceptScore W2184964767C192562407 @default.
- W2184964767 hasConceptScore W2184964767C204328495 @default.
- W2184964767 hasConceptScore W2184964767C2775981520 @default.
- W2184964767 hasConceptScore W2184964767C2776545253 @default.
- W2184964767 hasConceptScore W2184964767C32909587 @default.
- W2184964767 hasConceptScore W2184964767C49853544 @default.
- W2184964767 hasConceptScore W2184964767C55493867 @default.
- W2184964767 hasConceptScore W2184964767C59593255 @default.
- W2184964767 hasConceptScore W2184964767C62520636 @default.
- W2184964767 hasConceptScore W2184964767C8010536 @default.
- W2184964767 hasConceptScore W2184964767C86803240 @default.
- W2184964767 hasConceptScore W2184964767C91881484 @default.
- W2184964767 hasConceptScore W2184964767C96305047 @default.
- W2184964767 hasLocation W21849647671 @default.
- W2184964767 hasOpenAccess W2184964767 @default.
- W2184964767 hasPrimaryLocation W21849647671 @default.
- W2184964767 hasRelatedWork W2187810166 @default.
- W2184964767 hasRelatedWork W1967219301 @default.
- W2184964767 isParatext "false" @default.
- W2184964767 isRetracted "false" @default.
- W2184964767 magId "2184964767" @default.
- W2184964767 workType "article" @default.