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• W2184987419 abstract "Anchorage-dependent mammalian cells are used extensively in the production of biopharmaceuticals. cell seeding density, microcarrier concentration agitation rate was 40-60 rpm. A further increasing to 150 rpm resulted a significant reduction of growth with increasing the doubling time and reducing the exponential phase. An initial cell/bead inoculums of 25-30 ensured an even distribution of cells on the available microcarriers with a low proportion of unoccupied beads. An optimal growth rate occurred by an intermittent stirring regimen of 3 min stirring at 40 rpm per 30 min. Cell attachment to the electrostatic surface of the Cytodex 1 microcarrier was highly dependent on pH. This study showed that the rate of stirring influences greatly the growth and final yield of cells. Low agitation rate with initial intermittent stirring 40/30 minutes resulted in high cell densities and high of cell attachment. The growth rate of CHO cell increased with increasing concentration of cell inoculation, and therefore, the specific growth rate increases, too, quickly with increasing inoculation concentration." @default.
• W2184987419 created "2016-06-24" @default.
• W2184987419 creator A5048769713 @default.
• W2184987419 date "2010-01-01" @default.
• W2184987419 modified "2023-09-27" @default.
• W2184987419 title "Factors effects the Gr owth of Chinese Hamster Ovary (CHO) cell on Microcarriers Culture" @default.
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• W2184987419 hasPublicationYear "2010" @default.
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