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- W2184992105 abstract "Aim of the present study was to determine the FSH receptor expression pattern in buffalo preantral follicles (PFs). Mechanically isolated buffalo ovarian PFs were divided into two groups based on their size i.e. small (<150µm) and large PFs (150-300µm). Daily collection of follicles was divided into two storage temperature groups; one group was stored at -30°C whereas the second group of PFs was directly plunged into LN Storage 2. under -30°C was found to be ineffective, as no RNA could be extracted and recovered, however, sufficient amount of RNA could be extracted from the follicles stored in LN . The concentration of RNA obtained was 2 500 ng/µl, which was sufficient to carry out RT-PCR. cDNA was synthesized using Molony-Murine Leukemia virus transcriptase and amplified using specific oligonucleotide primers for buffalo FSH receptor. Specificity of the 2.184 kb amplicon was confirmed by RE digestion and nested PCR for amplification of an inner region corresponding to 486 bp was done. The purified PCR product was cloned to sequence the segment of the 2.184 kb insert. Compared with the available buffalo FSH receptor sequence using MegAlign package of DNA star program it revealed 100% homology. The same steps were repeated for large preantral follicles, identical results were observed as that of small PFs. Thus, there was an evidence for the presence of FSH receptors on small and large PFs. It is concluded that the FSH receptor system was present in the buffalo PFs having a diameter of less than 150 im and the expression continued in the PFs of 300 im diameters." @default.
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- W2184992105 date "2010-01-01" @default.
- W2184992105 modified "2023-09-22" @default.
- W2184992105 title "Cloning and Sequencing of FSH Receptor Gene from Buffalo Preantral Follicles" @default.
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