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• W2185017875 abstract "Structural variation in the genome is common due to insertions, deletions, duplications and rearrangements. However, little is known about the ways structural variants impact gene expression. Developmental genes are controlled by multiple regulatory sequence elements scattered over thousands of bases; developmental loci are therefore a good model to test the functional impact of structural variation on gene expression. Here, we measured the effect of rearranging two developmental enhancers from the even-skipped ( eve ) locus in Drosophila melanogaster blastoderm embryos. We systematically varied orientation, order, and spacing of the enhancers in transgenic reporter constructs and measured expression quantitatively at single cell resolution in whole embryos to detect changes in both level and position of expression. We found that the position of expression was robust to changes in locus organization, but levels of expression were highly sensitive to the spacing between enhancers and order relative to the promoter. Our data demonstrate that changes in locus architecture can dramatically impact levels of gene expression. To quantitatively predict gene expression from sequence, we must therefore consider how information is integrated both within enhancers and across gene loci." @default.
• W2185017875 created "2016-06-24" @default.
• W2185017875 creator A5015328271 @default.
• W2185017875 creator A5030626594 @default.
• W2185017875 creator A5044422375 @default.
• W2185017875 creator A5053074876 @default.
• W2185017875 creator A5067077903 @default.
• W2185017875 date "2014-05-14" @default.
• W2185017875 modified "2023-09-27" @default.
• W2185017875 title "Locus architecture affects mRNA expression levels in Drosophila embryos" @default.
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• W2185017875 doi "https://doi.org/10.1101/005173" @default.

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