FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2185046875> ?p ?o ?g. }

• W2185046875 abstract "The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the components present in the fractions VIIIA and VIIIB of Ts venom, in order to search for a novel toxin. The major isolated toxins were further investigated for macrophage modulation.The fractions VIIIA and VIIIB, obtained from Ts venom cation exchange chromatography, were rechromatographed on a C18 column (4.6 × 250 mm) followed by a reversed-phase chromatography using another C18 column (2.1 × 250 mm). The main eluted peaks were analyzed by MALDI-TOF and Edman's degradation and tested on macrophages.The previously described toxins Ts2, Ts3-KS, Ts4, Ts8, Ts8 propeptide, Ts19 Frag-II and the novel peptide Ts19 Frag-I were isolated from the fractions VIIIA and VIIIB. Ts19 Frag-I, presenting 58 amino acid residues, a mass of 6,575 Da and a theoretical pI of 8.57, shares high sequence identity with potassium channel toxins (KTx). The toxins Ts4, Ts3-KS and the partially purified Ts19 Frag-I did not produce cytotoxic effects on macrophage murine cells line (J774.1). On the other hand, Ts19 Frag-I induced the release of nitric oxide (NO) by macrophages, while Ts4 and Ts3-KS did not affect the NO production at the tested concentration (50 μg/mL). At the same concentration, Ts19 Frag-I and Ts3-KS increased the production of interleukin-6 (IL-6). Ts19 Frag-I and Ts4 did not induce the release of IL-10, IL-1β or tumor necrosis factor-α by macrophage cells using the tested concentration (50 μg/mL).We partially purified and determined the complete sequence and chemical/physical parameters of a new β-KTx, denominated Ts19 Frag-I. The toxins Ts4, Ts3-KS and Ts19 Frag-I showed no cytotoxicity toward macrophages and induced IL-6 release. Ts19 Frag-I also induced the release of NO, suggesting a pro-inflammatory activity." @default.
• W2185046875 created "2016-06-24" @default.
• W2185046875 creator A5007906188 @default.
• W2185046875 creator A5019054976 @default.
• W2185046875 creator A5022809598 @default.
• W2185046875 creator A5028386178 @default.
• W2185046875 creator A5045227946 @default.
• W2185046875 creator A5053596193 @default.
• W2185046875 creator A5056516134 @default.
• W2185046875 date "2015-12-01" @default.
• W2185046875 modified "2023-09-27" @default.
• W2185046875 title "Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom" @default.
• W2185046875 cites W1133779464 @default.
• W2185046875 cites W1531017848 @default.
• W2185046875 cites W1950374893 @default.
• W2185046875 cites W1966722182 @default.
• W2185046875 cites W1969827365 @default.
• W2185046875 cites W1970360839 @default.
• W2185046875 cites W1970388960 @default.
• W2185046875 cites W1973350402 @default.
• W2185046875 cites W1982730514 @default.
• W2185046875 cites W1984007496 @default.
• W2185046875 cites W1995330034 @default.
• W2185046875 cites W1997810187 @default.
• W2185046875 cites W2003197155 @default.
• W2185046875 cites W2006402662 @default.
• W2185046875 cites W2011695278 @default.
• W2185046875 cites W2017721112 @default.
• W2185046875 cites W2018195490 @default.
• W2185046875 cites W2026710934 @default.
• W2185046875 cites W2029429712 @default.
• W2185046875 cites W2039058376 @default.
• W2185046875 cites W2040210032 @default.
• W2185046875 cites W2042894552 @default.
• W2185046875 cites W2048211729 @default.
• W2185046875 cites W2051723572 @default.
• W2185046875 cites W2059441193 @default.
• W2185046875 cites W2060763741 @default.
• W2185046875 cites W2061080731 @default.
• W2185046875 cites W2067762246 @default.
• W2185046875 cites W2081801016 @default.
• W2185046875 cites W2083828993 @default.
• W2185046875 cites W2085474307 @default.
• W2185046875 cites W2100478793 @default.
• W2185046875 cites W2113396439 @default.
• W2185046875 cites W2114918609 @default.
• W2185046875 cites W2117718021 @default.
• W2185046875 cites W2130930961 @default.
• W2185046875 cites W2162347886 @default.
• W2185046875 cites W4235139664 @default.
• W2185046875 doi "https://doi.org/10.1186/s40409-015-0051-6" @default.
• W2185046875 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4666072" @default.
• W2185046875 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26628901" @default.
• W2185046875 hasPublicationYear "2015" @default.
• W2185046875 type Work @default.
• W2185046875 sameAs 2185046875 @default.
• W2185046875 citedByCount "11" @default.
• W2185046875 countsByYear W21850468752016 @default.
• W2185046875 countsByYear W21850468752017 @default.
• W2185046875 countsByYear W21850468752018 @default.
• W2185046875 countsByYear W21850468752019 @default.
• W2185046875 countsByYear W21850468752021 @default.
• W2185046875 countsByYear W21850468752022 @default.
• W2185046875 countsByYear W21850468752023 @default.
• W2185046875 crossrefType "journal-article" @default.
• W2185046875 hasAuthorship W2185046875A5007906188 @default.
• W2185046875 hasAuthorship W2185046875A5019054976 @default.
• W2185046875 hasAuthorship W2185046875A5022809598 @default.
• W2185046875 hasAuthorship W2185046875A5028386178 @default.
• W2185046875 hasAuthorship W2185046875A5045227946 @default.
• W2185046875 hasAuthorship W2185046875A5053596193 @default.
• W2185046875 hasAuthorship W2185046875A5056516134 @default.
• W2185046875 hasBestOaLocation W21850468751 @default.
• W2185046875 hasConcept C104317684 @default.
• W2185046875 hasConcept C167625842 @default.
• W2185046875 hasConcept C185592680 @default.
• W2185046875 hasConcept C2776676993 @default.
• W2185046875 hasConcept C2777367657 @default.
• W2185046875 hasConcept C2777884321 @default.
• W2185046875 hasConcept C2779281246 @default.
• W2185046875 hasConcept C2779448229 @default.
• W2185046875 hasConcept C43617362 @default.
• W2185046875 hasConcept C51724486 @default.
• W2185046875 hasConcept C55493867 @default.
• W2185046875 hasConcept C87933860 @default.
• W2185046875 hasConceptScore W2185046875C104317684 @default.
• W2185046875 hasConceptScore W2185046875C167625842 @default.
• W2185046875 hasConceptScore W2185046875C185592680 @default.
• W2185046875 hasConceptScore W2185046875C2776676993 @default.
• W2185046875 hasConceptScore W2185046875C2777367657 @default.
• W2185046875 hasConceptScore W2185046875C2777884321 @default.
• W2185046875 hasConceptScore W2185046875C2779281246 @default.
• W2185046875 hasConceptScore W2185046875C2779448229 @default.
• W2185046875 hasConceptScore W2185046875C43617362 @default.
• W2185046875 hasConceptScore W2185046875C51724486 @default.
• W2185046875 hasConceptScore W2185046875C55493867 @default.
• W2185046875 hasConceptScore W2185046875C87933860 @default.
• W2185046875 hasFunder F4320320997 @default.
• W2185046875 hasFunder F4320321091 @default.
• W2185046875 hasFunder F4320322025 @default.

• next