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• W2185068871 abstract "background. Natural populations of T. cruzi have been classified into six phylogenetic lineages or discrete typing units, T. cruzi I, IIa, IIb, IIc, IId, and IIe, believed to play a role in tissue tropism and disease pathogenesis. The impact of HIV infection in the T. cruzi genetic diversity in coinfected patients is a scarcely explored field of parasitology. Objective. To characterize parasitic lineages in clinical samples from patients co-infected with T. cruzi and HIV Materials and Methods. We analyzed blood and lesions samples from 25 patients residing in Argentina, namely 8 infants born to 7 HIV - T. cruzi co-infected mothers, 3 indeterminate adult chagasic patients with HIV co-infection and 7 presenting cerebral Chagas due to AIDS. Molecular diagnosis and monitoring of etiological treatment was carried out by PCR targeted to kinetoplastid (kDNA) and/or satellite sequences. T. cruzi lineages were identified by means of PCR targeted to the intergenic spacer of miniexon gene and 24s ribosomal ARN genes. To characterize the infra-lineage diversity, restriction fragment length polymorphism (RFLP) of KDNA amplicons was carried out. results. Out of the 7 co-infected mothers, two transmitted both HIV and T. cruzi to their siblings, four transmitted only T. cruzi. The remaining case was a pregnant woman with cerebral Chagas disease who entered into a coma being treated with benznidazole; she did not transmit congenital Chagas disease nor HIV to her newborn. Most bloodstream populations belonged to T. cruzi IId, with unique minicircle signatures for" @default.
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• W2185068871 date "2009-01-01" @default.
• W2185068871 modified "2023-09-24" @default.
• W2185068871 title "Trypanosoma cruzi discrete typing units in Chagas disease patients with HIV co-infection" @default.
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